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首页> 外文期刊>Bioprocess and Biosystems Engineering >[AuCl_4]~- and Fe~(3+)/[Fe(CN)_6]~(3-) ions-derivated immunosensing interface for electrochemical immunoassay of carcinoembryonic antigen in human serum
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[AuCl_4]~- and Fe~(3+)/[Fe(CN)_6]~(3-) ions-derivated immunosensing interface for electrochemical immunoassay of carcinoembryonic antigen in human serum

机译：[AuCl_4]〜-和Fe〜（3 +）/ [Fe（CN）_6]〜（3-）离子衍生的免疫传感界面用于人血清中癌胚抗原的电化学免疫分析

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摘要

[AuCl_4]~- was initially deposited by electrochemical reduction on a glassy carbon electrode (GCE) to form porous nanogold layer, then prussian blue (PB) was electrodeposited onto the as-prepared nanogold layer, and then secondary nanogold particles were fabricated again on the PB surface by electrochemical reduction for the immobilization of anti-CEA antibodies. The presence of double-layer porous gold nanoparticles enhanced the immobilized amount of biomolecules, and improved the sensitivity of the immunoassay. PB, as a good redox probe, was facile to electrochemical analysis and measurement. Under optimal conditions, the developed immunoassay exhibited dynamic range from 3.0 to 80.0 ng/mL with a detection limit of 0.9 ng/mL CEA (S/N = 3). Moreover, the selectivity, reproducibility and stability of the immu-nosensor were acceptable.
机译：首先通过电化学还原将[AuCl_4]〜-沉积在玻璃碳电极（GCE）上以形成多孔纳米金层，然后将普鲁士蓝（PB）电沉积到所制备的纳米金层上，然后在其上再次制造次级纳米金粒子。 PB表面通过电化学还原固定化抗CEA抗体。双层多孔金纳米颗粒的存在增强了生物分子的固定量，并提高了免疫测定的灵敏度。 PB作为良好的氧化还原探针，易于进行电化学分析和测量。在最佳条件下，开发的免疫测定法的动态范围为3.0至80.0 ng / mL，检出限为0.9 ng / mL CEA（S / N = 3）。而且，免疫传感器的选择性，再现性和稳定性是可接受的。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2010年第2期|179-185|共7页
  • 作者

    Kaige Liu; Ruo Yuan; Yaqin Chai; Dianping Tang; Haizhen An;

  • 作者单位

    Chongqing Key Laboratory of Analytical Chemistry,College of Chemistry and Chemical Engineering,Southwest University, 400715 Chongqing,People's Republic of China;

    Chongqing Key Laboratory of Analytical Chemistry,College of Chemistry and Chemical Engineering,Southwest University, 400715 Chongqing,People's Republic of China;

    Chongqing Key Laboratory of Analytical Chemistry,College of Chemistry and Chemical Engineering,Southwest University, 400715 Chongqing,People's Republic of China;

    Chongqing Key Laboratory of Analytical Chemistry,College of Chemistry and Chemical Engineering,Southwest University, 400715 Chongqing,People's Republic of China;

    Chongqing Key Laboratory of Analytical Chemistry,College of Chemistry and Chemical Engineering,Southwest University, 400715 Chongqing,People's Republic of China;

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  • 正文语种 eng
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  • 关键词

    amperometric immunosensor; carcinoembryonic antigen; ions derivation; prussian blue; nanogold;

    机译：安培免疫传感器癌胚抗原离子衍生普鲁士蓝;纳米金;

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