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首页> 外文期刊>Bioprocess and Biosystems Engineering >Single cell protein production from yacon extract using a highly thermosensitive and permeable mutant of the marine yeast Cryptococcus aureus G7a and its nutritive analysis
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Single cell protein production from yacon extract using a highly thermosensitive and permeable mutant of the marine yeast Cryptococcus aureus G7a and its nutritive analysis

机译：使用海洋酵母金黄色隐球菌G7a的高热敏性和可渗透性突变体从Yacon提取物中生产单细胞蛋白质及其营养分析

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摘要

The intracellular protein in the highly thermosensitive and permeable mutant can be easily released when they are incubated both in the low-osmolarity water and at the non-permissive temperature (usually 37 ℃). After the mutant was grown in the yacon extract for 45 h, the crude protein content in the highly thermosensitive and permeable mutant Z1 14 was 59.1% and over 61% of the total protein could be released from the cells treated at 37 ℃. The mutant cells grown in the yacon extract still contained high level of essential amino acids and other nutrients. This means that the yacon extract could be used as the medium for growth of the highly thermosensitive and permeable mutant which contained high content of crude protein.
机译：在低渗水和非允许温度（通常为37℃）中孵育时，高度热敏和可渗透的突变体中的胞内蛋白可以轻松释放。该突变体在yacon提取物中生长45 h后，高度热敏和可渗透的突变体Z1 14中的粗蛋白含量为59.1％，在37℃处理的细胞中可释放出总蛋白的61％以上。在Yacon提取物中生长的突变细胞仍然含有高水平的必需氨基酸和其他营养物质。这意味着孔提取物可以用作含有高含量粗蛋白的高度热敏且可渗透的突变体生长的培养基。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2010年第5期|P.549-556|共8页
  • 作者

    Chun-Hai Zhao; rnTong Zhang; rnZhen-Ming Chi; Zhe Chi; rnJing Li; rnXiang-Hong Wang;

  • 作者单位

    Key Laboratory of Marine Genetics, Gene Resource and Exploitation of Ministry of Education, Ocean University of China, Yushan Road, No. 5, Qingdao, China;

    rnKey Laboratory of Marine Genetics, Gene Resource and Exploitation of Ministry of Education, Ocean University of China, Yushan Road, No. 5, Qingdao, China;

    rnKey Laboratory of Marine Genetics, Gene Resource and Exploitation of Ministry of Education, Ocean University of China, Yushan Road, No. 5, Qingdao, China;

    rnKey Laboratory of Marine Genetics, Gene Resource and Exploitation of Ministry of Education, Ocean University of China, Yushan Road, No. 5, Qingdao, China;

    rnKey Laboratory of Marine Genetics, Gene Resource and Exploitation of Ministry of Education, Ocean University of China, Yushan Road, No. 5, Qingdao, China;

    rnKey Laboratory of Marine Genetics, Gene Resource and Exploitation of Ministry of Education, Ocean University of China, Yushan Road, No. 5, Qingdao, China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    highly thermosensitive and permeable mutant; cryptococcus aureus; single cell protein; yacon; inulin;

    机译：高度热敏和可渗透的突变体;金黄色隐球菌;单细胞蛋白yacon;菊粉;

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