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Enzymatic production of linalool esters in organic and solvent-free system

机译:在有机和无溶剂体系中酶促生成芳樟醇酯

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摘要

This work investigated the influence of temperature, enzyme concentration, substrates molar ratio, in the absence and presence of organic solvent, at two molar ratios of the substrates on the enzymatic production of linalil esters using the immobilized lipase Novozym 435 as catalyst, different acids and linalool and Ho-Sho essential oil as substrates. The best reaction conversion was obtained at the highest temperature (70 ℃), for both solvent free (3.81%) and with solvent addition (2.25%), for a solvent to substrates molar ratio of 2:1, enzyme concentration of 5 wt% and acid to alcohol molar ratio of 1:1. The reaction kinetics revealed that Ho-Sho essential oil afforded the greatest conversions when compared with pure linalool. Higher linalil esters production were achieved after 10 h reaction (5.58%) in 2:1 solvent to substrates molar ratio, with enzyme concentration of 5 wt%, at 70 ℃ and anhydride to alcohol molar ratio of 1:1 using Ho-Sho essential oil as substrate.
机译:这项工作研究了温度,酶浓度,底物摩尔比(在不存在和存在有机溶剂的情况下)的两种底物摩尔比对使用固定化脂肪酶Novozym 435作为催化剂,不同酸和芳樟醇和Ho-Sho精油为底物。在最高温度(70℃)下,对于无溶剂(3.81%)和添加溶剂(2.25%),对于溶剂与底物的摩尔比为2:1,酶浓度为5 wt%,都获得了最佳的反应转化率酸与醇的摩尔比为1:1。反应动力学表明,与纯芳樟醇相比,Ho-Sho精油的转化率最高。在70℃下,以2:1的溶剂与底物的摩尔比,酶浓度为5 wt%,在70℃和酸酐与醇的摩尔比为1:1的条件下,使用Ho-Sho必不可少的10 h反应(5.58%)后,可以实现更高的芳基尼尔酯产量油作为底物。

著录项

  • 来源
    《Bioprocess and Biosystems Engineering》 |2010年第5期|P.583-589|共7页
  • 作者单位

    Programa de Pos-Graduacao em Biotecnologia, Universidade de Caxias do Sul, Caxias do Sul, RS, Brazil Universidade Regional Integrada do Alto Uruguai e das Missoes, URI, Campus de Erechim, Av. Sete de Setembro, 1621, 99700-000 Erechim, RS, Brazil;

    rnUniversidade Regional Integrada do Alto Uruguai e das Missoes, URI, Campus de Erechim, Av. Sete de Setembro, 1621, 99700-000 Erechim, RS, Brazil;

    rnUniversidade Regional Integrada do Alto Uruguai e das Missoes, URI, Campus de Erechim, Av. Sete de Setembro, 1621, 99700-000 Erechim, RS, Brazil;

    rnUniversidade Regional Integrada do Alto Uruguai e das Missoes, URI, Campus de Erechim, Av. Sete de Setembro, 1621, 99700-000 Erechim, RS, Brazil;

    rnUniversidade Regional Integrada do Alto Uruguai e das Missoes, URI, Campus de Erechim, Av. Sete de Setembro, 1621, 99700-000 Erechim, RS, Brazil;

    rnUniversidade Regional Integrada do Alto Uruguai e das Missoes, URI, Campus de Erechim, Av. Sete de Setembro, 1621, 99700-000 Erechim, RS, Brazil;

    rnUniversidade Regional Integrada do Alto Uruguai e das Missoes, URI, Campus de Erechim, Av. Sete de Setembro, 1621, 99700-000 Erechim, RS, Brazil;

    rnUniversidade Regional Integrada do Alto Uruguai e das Missoes, URI, Campus de Erechim, Av. Sete de Setembro, 1621, 99700-000 Erechim, RS, Brazil;

    rnUniversidade Regional Integrada do Alto Uruguai e das Missoes, URI, Campus de Erechim, Av. Sete de Setembro, 1621, 99700-000 Erechim, RS, Brazil Programa de Pos-Graduacao em Biotecnologia, Universidade de Caxias do Sul, Caxias do Sul, RS, Brazil;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    linalool; Ho-Sho essential oil; novozym 435; biotransformation;

    机译:芳樟醇呵呵精油;新霉素435;生物转化;

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