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首页> 外文期刊>Bioprocess and Biosystems Engineering >Optimization of fermentation conditions for production of anti-TMV extracellular ribonuclease by Bacillus cereus using response surface methodology
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Optimization of fermentation conditions for production of anti-TMV extracellular ribonuclease by Bacillus cereus using response surface methodology

机译：响应面法优化蜡样芽孢杆菌生产抗TMV细胞外核糖核酸酶的发酵条件

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摘要

Bacillus cereus ZH14 was previously found to produce a new type of antiviral ribonuclease, which was secreted into medium and active against tobacco mosaic virus. In order to enhance the ribonuclease production, in this study the optimization of culture conditions using response surface methodology was done. The fermentation variables including culture temperature, initial pH, inoculum size, sucrose, yeast extract, MgSO_4·7H_2O, and KNO_3 were considered for selection of significant ones by using the Plackett-Burman design, and four significant variables (sucrose, yeast extract, MgSO_4·7H_2O, and KNO_3) were further optimized by a 2~4 factorial central composite design. The optimal combination of the medium constituents for maximum ribonuclease production was determined as 8.50 g/l sucrose, 9.30 g/l yeast extract, 2.00 g/l MgSO_4·7H_2O, and 0.62 g/l KNO_3. The enzyme activity was increased by 60%. This study will be helpful to the future commercial development of the new bacteria-based antiviral ribonuclease fermentation process.
机译：蜡状芽孢杆菌ZH14以前被发现产生一种新型的抗病毒核糖核酸酶，该酶被分泌到培养基中并具有抗烟草花叶病毒的活性。为了提高核糖核酸酶的产量，本研究使用响应面方法对培养条件进行了优化。通过使用Plackett-Burman设计，选择了包括发酵温度，初始pH，接种量，蔗糖，酵母提取物，MgSO_4·7H_2O和KNO_3在内的发酵变量来选择重要变量，以及四个重要变量（蔗糖，酵母提取物，MgSO_4） ·7H_2O和KNO_3）通过2〜4阶乘中心复合设计进一步优化。确定用于最大产量核糖核酸酶的培养基成分的最佳组合为8.50 g / l蔗糖，9.30 g / l酵母提取物，2.00 g / l MgSO_4·7H_2O和0.62 g / l KNO_3。酶活性增加了60％。这项研究将有助于新的基于细菌的抗病毒核糖核酸酶发酵工艺的未来商业发展。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2010年第6期|P.657-663|共7页
  • 作者

    Wen-Wen Zhou; Yun-Long He; Tian-Gui Niu; Jian-Jiang Zhong;

  • 作者单位

    Key Laboratory of Microbial Metabolism, Ministry of Education, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai 200240, China;

    College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua East Road, Beijing 100083, China;

    rnCollege of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua East Road, Beijing 100083, China;

    rnKey Laboratory of Microbial Metabolism, Ministry of Education, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai 200240, China;

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  • 正文语种 eng
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  • 关键词

    antiviral ribonuclease; bacillus cereus; fermentation; medium optimization; response surface methodology;

    机译：抗病毒核糖核酸酶;蜡状芽孢杆菌发酵中等优化;响应面法;

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