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Enzymatic synthesis of glutathione using yeast cells in two-stage reaction

机译：酵母细胞在两阶段反应中酶促合成谷胱甘肽

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In the present study, permeated yeast cells were used as the catalyst to synthesize glutathione. When waste cells of brewer's yeast were incubated with the three precursor amino acids and glucose for 36 h, 899 mg/L of glutathione were produced. To release the feedback inhibition of γ-glutamylcysteine synthetase caused by glutathione, two-stage reaction was adopted. In the first stage, glycine was omitted from the reaction mixture and only γ-glutamylcysteine was formed. Glycine was then added in the second stage, and 1,569 mg/L of glutathione were produced. The conditions of the two-stage reaction were optimized using Plackett-Burman design and response surface methodology. Under the optimized condition, commercially available baker's yeast produced 3,440 mg/L of glutathione in 30 h, and most of the produced glutathione was in the medium. The two-stage reaction could effectively reduce the feedback inhibition caused by glutathione, but degradation of glutathione was significant.
机译：在本研究中，渗透的酵母细胞用作合成谷胱甘肽的催化剂。将啤酒酵母的废细胞与三个前体氨基酸和葡萄糖孵育36小时后，产生了899 mg / L的谷胱甘肽。为了释放由谷胱甘肽引起的γ-谷氨酰半胱氨酸合成酶的反馈抑制，采用了两阶段反应。在第一阶段，反应混合物中省略了甘氨酸，仅形成了γ-谷氨酰半胱氨酸。然后在第二阶段添加甘氨酸，并产生了1,569 mg / L的谷胱甘肽。使用Plackett-Burman设计和响应面方法优化了两步反应的条件。在最佳条件下，市售面包酵母在30小时内产生了3,440 mg / L的谷胱甘肽，大部分产生的谷胱甘肽都在培养基中。两阶段反应可以有效减少谷胱甘肽引起的反馈抑制，但是谷胱甘肽的降解是显着的。

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