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首页> 外文期刊>Bioprocess and Biosystems Engineering >Effect Of Aeration And Agitation On The Protease Production By Staphylococcus Aureus Mutant Rc128 In A Stirred Tank Bioreactor
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Effect Of Aeration And Agitation On The Protease Production By Staphylococcus Aureus Mutant Rc128 In A Stirred Tank Bioreactor

机译：曝气和搅拌对搅拌罐生物反应器中金黄色葡萄球菌突变体Rc128产生蛋白酶的影响

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摘要

The modified rotating simplex method has been successfully used to determine the best combination of agitation rate and aeration rate for maximum production of extracellular proteases by Staphylococcus aureus mutant RC128, in a stirred tank bioreactor operated in a discontinuous way. This mutant has shown altered exoprotein production, specially enhanced protease production. Maximum production of proteases (15.28 UP/ml), measured using azocasein as a substrate, was obtained at exponential growth phase when the bioreactor was operated at 300 rpm and at 2 vvm with a volumetric oxygen transfer coefficient (K_La) of 175.75 h~(-1). These conditions were found to be more suitable for protease production.
机译：改良的旋转单纯形法已成功地用于确定搅拌速率和通气速率的最佳组合，以在不连续操作的搅拌罐生物反应器中通过金黄色葡萄球菌突变体RC128最大程度地生产胞外蛋白酶。该突变体显示出改变的外蛋白产生，特别是增强的蛋白酶产生。当生物反应器在300 rpm和2 vvm的条件下以体积氧转移系数（K_La）为175.75 h〜（ -1）。发现这些条件更适合于蛋白酶的生产。

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