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首页> 外文期刊>Bioprocess and Biosystems Engineering >Ethanol And Acetate Production By Clostridium Ljungdahlii And Clostridium Autoethanogenum Using Resting Cells
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Ethanol And Acetate Production By Clostridium Ljungdahlii And Clostridium Autoethanogenum Using Resting Cells

机译：静息梭状芽胞杆菌和自产乙醇梭菌生产乙醇和乙酸盐

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摘要

Combined gasification and fermentation technologies can potentially produce biofuels from renewable biomass. Gasification generates synthesis gas consisting primarily of CO, CO_2, H_2, N_2, with smaller amounts of CH_4, NO_x, O_2, C_2 compounds, ash and tars. Several anaerobic bacteria species can ferment bottled mixtures of pure synthesis gas constituents. However, there are challenges to maintaining culture viability of synthesis gas exposed cells. This study was designed to enhance culture stability and improve ethanol-to-acetate ratios using resting (non-growing) cells in synthesis gas fermentation. Resting cell states were induced in autotrophic Clostridium ljungdahlii cultures with minimal ethanol and acetate production due to low metabolic activity compared to growing cell production levels of 5.2 and 40.1 mM of ethanol and acetate. Clostridium autoethanogenum cultures were not induced into true resting states but did show improvement in total ethanol production (from 5.1 mM in growing cultures to 9.4 in one nitrogen-limited medium) as well as increased shifts in ethanol-to-acetate production ratios.
机译：结合的气化和发酵技术可以潜在地利用可再生生物质生产生物燃料。气化产生主要由CO，CO_2，H_2，N_2组成的合成气，以及少量CH_4，NO_x，O_2，C_2化合物，灰分和焦油。几种厌氧菌可以发酵纯合成气成分的瓶装混合物。然而，维持合成气体暴露的细胞的培养活力存在挑战。这项研究旨在通过在合成气发酵中使用静止（非生长）细胞来增强培养物稳定性并提高乙醇与乙酸盐的比率。在自养的梭菌梭状芽胞杆菌培养物中，由于代谢活性低，相比于不断增长的5.2和40.1 mM乙醇和乙酸盐的细胞生产水平，可以诱导乙醇和乙酸盐的产量最少的静止细胞状态。梭状芽孢杆菌培养物没有被诱导成真正的静止状态，但确实显示出总乙醇产量的提高（从生长的培养物中的5.1 mM到一种氮限制培养基中的9.4），以及乙醇与乙酸盐的生产比率的增加。

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