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首页> 外文期刊>Bioprocess and Biosystems Engineering >Electrochemical nitrite biosensor based on the immobilization of hemoglobin on an electrode modified by multiwall carbon nanotubes and positively charged gold nanoparticle
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Electrochemical nitrite biosensor based on the immobilization of hemoglobin on an electrode modified by multiwall carbon nanotubes and positively charged gold nanoparticle

机译：基于血红蛋白固定在多壁碳纳米管和带正电的金纳米粒子修饰的电极上的电化学亚硝酸盐生物传感器

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摘要

The report is on an electrochemical biosensor with remarkably improved sensitivity toward nitrite. In this strategy, positively charged gold nanoparticle (PCNA) is used in combination with multiwall carbon nanotubes (MWCNT) by electrostatic adsorption for fabricating PCNA/MWCNT films. Then hemoglobin (Hb) biocatalyst will easily be attached to the surface of the combination films aforementioned. After that, the Hb/PCNA films are immobilized onto the Hb/PCNA/MWCNT films through layer-by-layer assembly technique. The (Hb/PCNA)_2/ MWNT/GC electrode thus prepared exhibits enhanced electrocatalytic behavior to the reduction of nitrite at -0.10 V versus SCE in 0.05 M H_2SO_4 solution. On condition of the low detecting potential and low pH, interference caused by direct electrochemical oxidation or oxidizable substances can be prevented. Therefore, the modified electrode shows fast response time, very high sensitivity, good selectivity and stability. The current response of the sensor increases linearly with nitrite concentration from a range of 3.6 × 10~(-6) to 3.0 × 10~(-3) M with a detection limit(S/N = 3) of 9.6 × 10~(-7) M.
机译：该报告是关于一种电化学生物传感器，对亚硝酸盐的敏感性显着提高。在这种策略中，通过静电吸附将带正电的金纳米颗粒（PCNA）与多壁碳纳米管（MWCNT）结合使用，以制造PCNA / MWCNT膜。然后，血红蛋白（Hb）生物催化剂将很容易附着在上述结合膜的表面。之后，通过逐层组装技术将Hb / PCNA膜固定在Hb / PCNA / MWCNT膜上。如此制备的（Hb / PCNA）_2 / MWNT / GC电极相对于0.05M H_2SO_4溶液中的SCE，在-0.10 V下对亚硝酸盐的还原表现出增强的电催化行为。在低检测电位和低pH的条件下，可以防止直接电化学氧化或可氧化物质引起的干扰。因此，改进的电极显示出快速的响应时间，非常高的灵敏度，良好的选择性和稳定性。传感器的电流响应随亚硝酸盐浓度从3.6×10〜（-6）到3.0×10〜（-3）M的范围呈线性增加，检测极限（S / N = 3）为9.6×10〜（ -7）M.

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2009年第4期|485-492|共8页
  • 作者

    Lingyan Zhang; Min Yi;

  • 作者单位

    Forensic Identification Center, College of Criminal Investigation. Southwest University of Political Science and Law, 401120 Chongqing, China Chongqing Institutions of Higher Learning Center of Forensic Science Engineering and Research, Forensic Identification Center, Southwest University of Political Science and Law, 401120 Chongqing, China;

    Forensic Identification Center, College of Criminal Investigation. Southwest University of Political Science and Law, 401120 Chongqing, China Chongqing Institutions of Higher Learning Center of Forensic Science Engineering and Research, Forensic Identification Center, Southwest University of Political Science and Law, 401120 Chongqing, China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    positively charged gold nanoparticle; multiwall carbon nanolubes; electrocatalytic; hemoglobin; nitrite;

    机译：带正电的金纳米粒子;多壁碳纳米润滑剂;电催化血红蛋白亚硝酸盐;

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