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首页> 外文期刊>Bioprocess and Biosystems Engineering >Amperometric hydrogen peroxide biosensor based on covalently immobilizing thionine as a mediator
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Amperometric hydrogen peroxide biosensor based on covalently immobilizing thionine as a mediator

机译：基于共价固定化硫氨酸作为介体的安培过氧化氢生物传感器

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摘要

A novel amperometric hydrogen peroxide biosensor based on the immobilization of hemoglobin on the 2,6-pyridinedicarboxylic acid (PDC) polymer, thionine and nano-Au was successfully fabricated. In this strategy, PDC polymer acted as the matrices to covalently immobilize the thionine, and then hemoglobin was successfully adsorbed on the nano-Au which was electro-deposited on to thionine modified electrode surface. The preparation process of modified electrode was characterized with electrochemical impedance spectroscopy and atomic force microscope. The analytical performance of proposed biosensor toward H_2O_2 was investigated by cyclic voltammetry and chronoampe-rometry. The resulted biosensor exhibited fast amperometric response (within 6 s) to H_2O_2, and linear range was from 9.1 μM to 5.0 mM with the detection limit of 2.6 μM (S/ N = 3). The apparent Michaelis-Menten constant (A_M~(app)) was evaluated to be 3.2 mM. Furthermore, the resulted biosensor showed good stability and reproducibility.
机译：基于2,6-吡啶二甲酸（PDC）聚合物，硫氨酸和纳米金的血红蛋白固定化，成功地制备了一种新型的安培过氧化氢生物传感器。在这种策略中，PDC聚合物充当共价固定亚硫氨酸的基质，然后将血红蛋白成功吸附在纳米Au上，然后将其电沉积到亚硫氨酸修饰的电极表面。通过电化学阻抗谱和原子力显微镜对改性电极的制备过程进行了表征。通过循环伏安法和计时电流法研究了拟议的生物传感器对H_2O_2的分析性能。所得生物传感器对H_2O_2表现出快速的安培响应（在6 s内），线性范围为9.1μM至5.0 mM，检测极限为2.6μM（S / N = 3）。表观Michaelis-Menten常数（A_M〜（app））经评估为3.2 mM。此外，所得生物传感器显示出良好的稳定性和再现性。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2009年第4期|537-544|共8页
  • 作者

    Liping Ma; Ruo Yuan; Yaqin Chai; Shihong Chen; Shujuan Ling;

  • 作者单位

    Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, 400715 Chongqing. China;

    Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, 400715 Chongqing. China;

    Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, 400715 Chongqing. China;

    Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, 400715 Chongqing. China;

    Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, 400715 Chongqing. China;

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  • 正文语种 eng
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  • 关键词

    2,6-pyridinedicarboxylic acid; thionine; nano-au; hemoglobin; biosensor;

    机译：2,6-吡啶二羧酸;硫氨酸纳米金血红蛋白生物传感器;

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