...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioprocess and Biosystems Engineering >Optimization of extracellular psychrophilic alkaline lipase produced by marine Pseudomonas sp. (MSI057)
【24h】

Optimization of extracellular psychrophilic alkaline lipase produced by marine Pseudomonas sp. (MSI057)

机译：海洋假单胞菌产生的细胞外嗜冷碱性脂肪酶的优化。 （MSI057）

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An endosymbiotic Pseudomonas sp. (MSI057), which could produce high yields of lipase, was isolated from marine sponge Dendrilla nigra, collected from the peninsular coast of India. Maximum production of enzyme was obtained in minimal medium supplemented with 1% tributyrin. Catabolite repression was observed when the medium was supplemented with readily available carbon sources. The optimum temperature and pH for the enzyme production was 30 °C and 9.0, respectively. The enzyme exhibited maximum activity in pH range of 8-9 with an optimum pH 9.0. The activity of purified enzyme was optimum at 37 °C and showed 80% activity at 20 °C and the enzyme activity decreased dramatically above 50 °C. Based on the present findings, the enzyme was characterized as psychrophilic alkaline lipase, which can be developed for industrial applications.
机译：内共生假单胞菌（MSI057）可以产生高脂肪酶，是从印度半岛沿岸收集的海生海绵Dendrilla nigra中分离出来的。在补充了1％三丁酸甘油酯的基本培养基中获得了最大的酶产量。当培养基中补充有容易获得的碳源时，观察到分解代谢物的抑制。酶生产的最佳温度和pH分别为30°C和9.0。该酶在8-9的pH范围内表现出最大活性，最适pH值为9.0。纯化的酶的活性在37°C时最佳，在20°C时显示80％的活性，在50°C以上时酶的活性急剧下降。基于目前的发现，该酶被表征为嗜碱性脂肪酶，可以开发用于工业应用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号