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首页> 外文期刊>Bioprocess and Biosystems Engineering >A mediator-free amperometric hydrogen peroxide biosensor based on HRP immobilized on a nano-Au/poly 2,6-pyridinediamine-coated electrode
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A mediator-free amperometric hydrogen peroxide biosensor based on HRP immobilized on a nano-Au/poly 2,6-pyridinediamine-coated electrode

机译：基于HRP的无介体电流型过氧化氢生物传感器固定在纳米Au /聚2,6-吡啶二胺涂层电极上

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摘要

A mediator-free amperometric hydrogen peroxide biosensor was prepared by immobilizing horseradish peroxidase (HRP) enzyme on colloidal Au modified platinum (Pt) wire electrode, which was modified by poly 2,6-pyridinediamine (pPA). The modified process was characterized by electrochemical impedance spectroscopy (EIS), and the electrochemical characteristics of the biosensor were studied by cyclic voltammetry, linear sweep voltammetry and chrono-amperometry. The biosensor displayed an excellent electrocatalytical response to reduction of H_2O_2 without the aid of an electron mediator, the linear range was 4.2 x 10~(-7)-1.5 x 10~(-3) mol/L (r = 0.9977), with a detection limit of 1.4 x 10~(-7) mol/L. Moreover, the performance and factors influencing the resulted biosensor were studied in detail. The studied biosensor exhibited permselectivity, good stability and good fabrication reproducibility.
机译：通过将辣根过氧化物酶（HRP）酶固定在胶体Au修饰的铂（Pt）丝电极上制备无介体的安培型过氧化氢生物传感器，该电极经聚2,6-吡啶二胺（pPA）修饰。通过电化学阻抗谱（EIS）对改进的工艺进行了表征，并通过循环伏安法，线性扫描伏安法和计时电流法研究了生物传感器的电化学特性。该生物传感器在不借助电子介体的情况下显示出对H_2O_2还原的出色的电催化响应，线性范围为4.2 x 10〜（-7）-1.5 x 10〜（-3）mol / L（r = 0.9977），检出限为1.4 x 10〜（-7）mol / L。此外，详细研究了性能和影响所得生物传感器的因素。所研究的生物传感器表现出了选择性，良好的稳定性和良好的制造再现性。

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