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首页> 外文期刊>Bioprocess and Biosystems Engineering >Cytotoxicity of purified cassava linamarin to a selected cancer cell lines
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Cytotoxicity of purified cassava linamarin to a selected cancer cell lines

机译：纯化木薯亚麻苦素对选定癌细胞系的细胞毒性

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Cassava (Manihot esculenta Crantz) is a known source of linamarin, but difficulties associated with its isolation have prevented it from being exploited as a major source. A batch adsorption process using activated carbon proved successful in its isolation, with ultrafiltration playing a pivotal role in its purification. Thirty-two minutes of contact time was required for 60 g of extract, yielding 1.7 g of purified product. Picrate paper, infra-red and ~1HNMR analysis confirmed the presence and structure of linamarin. Cytotoxic effects of linamarin on MCF-7, HT-29 and HL-60 cells were determined using the MTT assay. Cytotoxic effects were significantly increased in the presence of lin amarase (β-glucosidase), with a 10-fold decrease in the IC_(50) values obtained for HL-60 cells. This study thus describes a method for the isolation and purification of linamarin from cassava, as well as its cytotoxicity potential.
机译：木薯（Manihot esculenta Crantz）是亚麻苦素的已知来源，但是由于其分离所带来的困难使它不能被用作主要来源。事实证明，使用活性炭的分批吸附工艺可成功分离，超滤在其纯化中起着关键作用。 60克提取物需要三十二分钟的接触时间，得到1.7克纯化产物。苦味纸，红外和〜1HNMR分析证实了亚麻苦素的存在和结构。使用MTT测定法测定了亚麻苦碱对MCF-7，HT-29和HL-60细胞的细胞毒性作用。在存在lin amarase（β-葡萄糖苷酶）的情况下，细胞毒性作用显着增强，HL-60细胞获得的IC_（50）值降低了10倍。因此，这项研究描述了一种从木薯中分离纯化亚麻苦素的方法及其潜在的细胞毒性。

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