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首页> 外文期刊>Bioprocess and Biosystems Engineering >Novel immunoassay for carcinoembryonic antigen based on protein A-conjugated immunosensor chip by surface plasmon resonance and cyclic voltammetry
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Novel immunoassay for carcinoembryonic antigen based on protein A-conjugated immunosensor chip by surface plasmon resonance and cyclic voltammetry

机译：基于表面等离子体激元共振和循环伏安法的蛋白A偶联免疫传感器芯片对癌胚抗原的免疫测定

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摘要

In this study, an immunosensor chip utilizing surface plasmon resonance (SPR) and cyclic voltammetry (CV) was fabricated for detecting carcinoembryonic antigen (CEA). Specifically, we applied in parallel an SPR instrument and a CV device to monitor the assembly of carcinoembryonic antibody (anti-CEA) on a protein A-conjugated surface and the subsequent ligand reaction. The immunosensor chips were constructed by various concentrations of protein A. To determine the surface characteristics of different self-assembly mono-layers (SAMs), several quantitative and kinetic measurements were carried out. The extent of immobilization of anti-CEA and the immune response qf anti-CEA antibody against CEA were measured using the SPR instrument and CV device. The terminal functional groups of protein A have different effects on the adsorption and covalent binding of immunoprotein depending on the steric hindrance. Through the parallel measurements, we demonstrate that SPR and CV are sensitive to measure the antigen-antibody binding capacity.
机译：在这项研究中，利用表面等离振子共振（SPR）和循环伏安法（CV）制作了一种免疫传感器芯片，用于检测癌胚抗原（CEA）。具体而言，我们并行应用了SPR仪器和CV装置，以监测在蛋白A偶联表面上的癌胚抗体（抗CEA）的组装以及随后的配体反应。免疫传感器芯片由各种浓度的蛋白A构成。为了确定不同自组装单层（SAM）的表面特征，进行了一些定量和动力学测量。使用SPR仪器和CV设备测量抗CEA的固定化程度和针对CEA的免疫应答qf抗CEA抗体。取决于空间位阻，蛋白A的末端官能团对免疫蛋白的吸附和共价结合有不同的影响。通过并行测量，我们证明了SPR和CV对测量抗原-抗体结合能力很敏感。

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