...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioprocess and Biosystems Engineering >Breakthrough performance of linear-DNA on ion-exchange membrane columns
【24h】

Breakthrough performance of linear-DNA on ion-exchange membrane columns

机译：线性DNA在离子交换膜色谱柱上的突破性能

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Breakthrough performance of linear-DNA adsorption on ion-exchange membrane columns was theoretically and experimentally investigated using batch and fixed-bed systems. System dispersion curves showed the absence of flow non-idealities in the experimental arrangement. Breakthrough curves were not significantly affected by flow-rate or inlet solution concentration. In the theoretical analysis a model was integrated by the serial coupling of the membrane transport model and the system dispersion model. A transport model that considers finite kinetic rate and column dispersed flow was used in the study. A simplex optimization routine coupled to the solution of the partial differential model equations was employed to estimate the maximum adsorption capacity constant, the equilibrium desorption constant and the forward interaction rate-constant, which are the parameters of the membrane transport model. Through this approach a good prediction of the adsorption phenomena is obtained for inlet concentrations and flow rates greater than 0.2 mg/ml and 0.16 ml/min.
机译：在线性和离子交换膜柱上吸附线性蛋白质的突破性能进行了理论和实验上使用批处理和固定床系统的研究。系统分散曲线表明，在实验装置中没有流动非理想性。穿透率曲线不受流速或入口溶液浓度的显着影响。在理论分析中，通过膜传输模型和系统分散模型的串联耦合来集成模型。在研究中使用了考虑有限动力学速率和色谱柱分散流的传输模型。采用单纯形优化程序，并结合偏微分模型方程的解来估计最大吸附容量常数，平衡解吸常数和正向相互作用速率常数，它们是膜传输模型的参数。通过这种方法，可以很好地预测进口浓度和流速大于0.2 mg / ml和0.16 ml / min的吸附现象。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号