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On-line optimization of glutamate production based on balanced metabolic control by RQ

机译：基于RQ平衡代谢控制的谷氨酸生产在线优化

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In glutamate fermentations by Corynebacte-rium glutamicum, higher glutamate concentration could be achieved by constantly controlling dissolved oxygen concentration (DO) at a lower level; however, by-product lactate also severely accumulated. The results of analyzing activities changes of the two key enzymes, glutamate and lactate dehydrogenases involved with the fermentation, and the entire metabolic network flux analysis showed that the lactate overproduction was because the metabolic flux in TCA cycle was too low to balance the glucose glycolysis rate. As a result, the respiratory quotient (RQ) adaptive control based "balanced metabolic control" (BMC) strategy was proposed and used to regulate the TCA metabolic flux rate at an appropriate level to achieve the metabolic balance among glycolysis, glutamate synthesis, and TCA metabolic flux. Compared with the best results of various DO constant controls, the BMC strategy increased the maximal glutamate concentration by about 15% and almost completely repressed the lactate accumulation with competitively high glutamate productivity.
机译：在谷氨酸棒杆菌发酵谷氨酸的过程中，可以通过将溶解氧浓度（DO）始终控制在较低水平来实现更高的谷氨酸浓度。但是，副产物乳酸也大量积累。分析与发酵有关的两个关键酶谷氨酸和乳酸脱氢酶的活性变化的结果，以及整个代谢网络通量分析表明，乳酸的过量生产是由于TCA循环中的代谢通量太低而无法平衡葡萄糖糖酵解速率。因此，提出了基于呼吸商（RQ）自适应控制的“平衡代谢控制”（BMC）策略，并将其用于调节TCA代谢通量率的适当水平，以实现糖酵解，谷氨酸合成和TCA之间的代谢平衡。代谢通量。与各种DO恒定对照的最佳结果相比，BMC策略将最大谷氨酸浓度提高了约15％，并且几乎完全抑制了乳酸积累，并具有竞争性的高谷氨酸生产率。

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