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首页> 外文期刊>Bioprocess and Biosystems Engineering >Soft material-based microculture system having air permeable cover sheet for the protoplast culture of Nicotiana tabacum
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Soft material-based microculture system having air permeable cover sheet for the protoplast culture of Nicotiana tabacum

机译：具有透气性盖片的基于软材料的微培养系统，用于烟草的原生质体培养

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In plant cell culture, the delivery of nutrition and gas (mainly oxygen) to the cells is the most important factor for viability. In this paper, we propose a polydimethylsiloxane (PDMS)-based microculture system that is designed to have good aeration. PDMS is known to have excellent air permeability, and through the experimental method, we investigated the relation between the degree of air delivery and the thickness of the PDMS sheet covering the culture chamber. We determined the proper thickness of the cover sheet, and cultured protoplasts of Nicotiana tabacum in a culture chamber covered with a PDMS sheet having thickness of 400 urn. The cells were successfully divided, and lived well inside the culture chamber for 10 days. In addition, protoplasts were cultured inside the culture chambers covered with the cover glass and the PDMS sheet, respectively, and the microcolonies were formed well inside the PDMS covered chamber after 10 days.
机译：在植物细胞培养中，营养和气体（主要是氧气）向细胞的输送是生存力的最重要因素。在本文中，我们提出了一种基于聚二甲基硅氧烷（PDMS）的微培养系统，该系统旨在具有良好的通气性。 PDMS具有出色的透气性，并且通过实验方法，我们研究了空气输送程度与覆盖培养室的PDMS薄板厚度之间的关系。我们确定了覆盖片的适当厚度，并在覆盖有厚度为400微米的PDMS片的培养室中培养了烟草的原生质体。细胞成功分裂，并在培养室内良好生活了10天。另外，原生质体分别在覆盖有盖玻片和PDMS片的培养室内进行培养，并且在10天后在PDMS覆盖的室内充分形成微菌落。

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