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首页> 外文期刊>Bioprocess and Biosystems Engineering >Kinetic and enzymatic adsorption model in a recirculation hollow-fibre bioreactor
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Kinetic and enzymatic adsorption model in a recirculation hollow-fibre bioreactor

机译：循环中空纤维生物反应器中的动力学和酶吸附模型

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A general procedure has been developed to model the behaviour of enzymatic reactions in a membrane bioreactor. This procedure unifies the kinetics of the reaction and the adsorption of the enzyme or enzymatic complexes on the membrane, enabling the selection of the most appropriate kinetic model. The general procedure proposed has been particularized and applied to experimental results obtained with two enzymatic reactions carried out in a hollow-fibre reactor, enzymatic hydrolysis of lactose by β-galactosidase and glucose-fructose isomerization by glucose isomerase. The application of the general model has allowed us to determine the mechanism of the reaction for both kinetic reactions, assuming the adsorption of the enzymatic complex EGa for lactose hydrolysis and the adsorption of the free enzyme onto the membrane for glucose-fructose isomerization.
机译：已经开发出通用程序来模拟膜生物反应器中酶促反应的行为。该程序统一了反应动力学和酶或酶复合物在膜上的吸附，从而能够选择最合适的动力学模型。提出的一般程序已经具体化，并应用于在中空纤维反应器中进行的两次酶促反应，β-半乳糖苷酶对乳糖的酶水解和葡萄糖异构酶对葡萄糖-果糖异构化的实验结果。通用模型的应用使我们能够确定两个动力学反应的反应机理，假定酶复合物EGa吸附用于乳糖水解，游离酶在膜上吸附用于葡萄糖-果糖异构化。

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