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Bioreactor hydrodynamic effect on Escherichia coli physiology: experimental results and stochastic simulations

机译：生物反应器对大肠杆菌生理的流体动力学影响：实验结果和随机模拟

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摘要

A microorganism circulating in a bioreactor can be submitted to hydrodynamic conditions inducing a significant effect on its physiology. The mixing time exhibited by the stirred bioreactor and the circulation of microorganisms are both involved in this reacting system. The mixing component determines the intensity of the concentration gradient and the circulation component determines the way in which the microorganism is exposed to this gradient. These two components linked to the experimental evaluation of microbial physiology can be analysed by a structured stochastic model in the case of a partitioned or "scale-down" reactor (SDR). A stochastic model indeed enables to simulate the mixing process as well as the circulation of microorganisms in SDRs. The superimposition of mixing and circulation processes determines the concentration profile experienced by a microorganism in the reactor. In the present case, the glucose concentration experienced by Escherichia coli has been modelled during a fed-batch culture. In this context, the use of a stochastic hydrodynamic model has permitted to point out an interesting feed pulse retardant effect in the SDRs. Nevertheless, the metabolic response of E. coli is not easy to interpret because of the possible simultaneous developments of overflow metabolism and mixed acid fermentation induced by the strong glucose concentration in the reactor.
机译：在生物反应器中循环的微生物可以经受流体动力学条件，从而对其生理机能产生重大影响。搅拌的生物反应器显示的混合时间和微生物的循环都参与该反应系统。混合成分决定浓度梯度的强度，循环成分决定微生物暴露于该梯度的方式。在分区或“按比例缩小”反应器（SDR）的情况下，可以通过结构化随机模型分析与微生物生理学实验评估相关的这两个组件。随机模型的确可以模拟SDR中的混合过程以及微生物的循环。混合和循环过程的叠加确定了反应器中微生物所经历的浓度分布。在当前情况下，已经在分批补料培养过程中模拟了大肠杆菌所经历的葡萄糖浓度。在这种情况下，使用随机流体动力学模型可以指出SDR中有趣的进料脉冲延迟效应。然而，由于反应器中高浓度的葡萄糖可能导致溢流代谢和混合酸发酵同时发展，因此大肠杆菌的代谢反应不易解释。

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