Bounded water kinetic model of β-galactosidase in reverse micelles

C. Will Chen; Chao-Chih Ou-Yang

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Bounded water kinetic model of β-galactosidase in reverse micelles

机译：反胶束中β-半乳糖苷酶的有界水动力学模型

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In the present investigation, β-galactosidase was solubilized into Aerosol OT (AOT)/isooctane reverse micelles. Kinetic data for the hydrolysis of o-ni-trophenyl-β-D-galactopyranoside (ONPG) at different pH values and molar ratios of water to AOT (W_o) were collected. It was observed that the usual kinetic model used for β-galactosidase catalysis in aqueous systems failed to represent the experimental data. A bounded water model, however, showed a better correlation between enzymatic activity and W_o. In contrast to the aqueous system, controlling the water concentration in the reverse micelles allows the rate constants for the reaction between water molecules and glycosyl-enzyme complexes to be evaluated.

机译：在本研究中，β-半乳糖苷酶被增溶成气溶胶OT（AOT）/异辛烷反胶束。收集了在不同pH值和水与AOT的摩尔比（W_o）下邻硝基三苯基-β-D-吡喃半乳糖苷（ONPG）水解的动力学数据。观察到用于水性系统中的β-半乳糖苷酶催化的常用动力学模型不能代表实验数据。但是，有界水模型显示了酶活性与W_o之间的更好的相关性。与水性体系相反，控制反胶束中的水浓度可以评估水分子与糖基酶复合物之间反应的速率常数。

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来源
《Bioprocess and Biosystems Engineering》 |2004年第5期|p.307-313|共7页
作者
C. Will Chen; Chao-Chih Ou-Yang;
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作者单位

Department of Bioengineering, Tatung University, 40 Chungshan N. Rd., 3rd Sec., Taipei, Taiwan, Republic of China;

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原文格式 PDF
正文语种 eng
中图分类生物工程学（生物技术）;
关键词
bounded water model; reverse micelle; β-galactosidase;

机译：有界水模型;反胶束;β-半乳糖苷酶;

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专利

1. Modelling the alcoholysis reaction of #beta#-galactosidase with butanol in reverse micelles [J] . A. Moreno-Beltran, L. Salgado, R. Vazquez-Duhalt, Journal of molecular catalysis, B. Enzymatic . 1999,第1a2期

机译：模拟反胶束中＃beta＃-半乳糖苷酶与丁醇的醇解反应
2. Intramolecular charge transfer at reverse micelle-water pool interface: p-N,N-dimethylaminobenzoic acid in AOT/cyclohexane/water reverse micelle [J] . Jiang YB., Jin MG. Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy . 2000,第4期

机译：反向胶束-水池界面处的分子内电荷转移：AOT /环己烷/水反向胶束中的对-N，N-二甲基氨基苯甲酸
3. Kinetics of cutinase catalyzed transesterification in AOT reversed micelles: modeling of a batch stirred tank reactor [J] . C.M.L. Carvalho, M.R. Aires-Barros J.M.S. Cabral, J.M.S. Cabral Journal of Biotechnology . 2000,第1期

机译：AOT逆胶束中角质酶催化的酯交换反应动力学：间歇搅拌釜反应器模型
4. Study on Kinetics of Protein Forward Extraction from Quinoa by AOT/Isooctane Reverse Micelles System [C] . Xiaoyong Wu, Yanxia Sun, Yujie Shi, International Conference on Modelling, Simulation and Applied Mathematics . 2018

机译：AOT / ISOOCTANE反转胶束系统藜氨基蛋白前萃取动力学研究
5. Reverse micelle encapsulation as a biophysical technique to study the effects of confinement and low temperature on water and protein. [D] . Simorellis, Alana Kristin. 2007

机译：反胶束封装作为一种生物物理技术来研究限制和低温对水和蛋白质的影响。
6. Kinetic models in reverse micelles. [O] . R Bru, A Sánchez-Ferrer, F García-Carmona 1995

机译：反向胶束的动力学模型。
7. Kinetic models in reverse micelles. [O] . Bru, R, Sánchez-Ferrer, A, García-Carmona, F 1995

机译：反向胶束的动力学模型。

Bounded water kinetic model of β-galactosidase in reverse micelles

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