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Conversion of paper sludge to ethanol in a semiontinuous solids-fed reactor

机译:在半连续固体进料反应器中将纸污泥转化为乙醇

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Conversion of paper sludge to ethanol was investigated with the objective of operating under conditions approaching those expected of an industrial process. Major components of the bleached Kraft sludge studied were glucan (62 wt.%, dry basis), xylan (11.5%), and minerals (17%). Complete recovery of glucose during compositional analysis required two acid hydrolysis treatments rather than one. To avoid the difficulty of mixing unreacted paper sludge, a semicontinuous solids-fed laboratory bioreactor system was developed. The system featured feeding at 12-h intervals, a residence time of 4 days, and cellulase loading of 15 to 20 FPU/g cellulose. Sludge was converted to ethanol using simultaneous saccharification and fermentation (SSF) featuring a -glucosidase-supplemented commercial cellulase preparation and glucose fermentation by Saccharomyces cerevisiea. SSF was carried out for a period of 4 months in a first-generation system, resulting in an average ethanol concentration of 35 g/L. However, steady state was not achieved and operational difficulties were encountered. These difficulties were avoided in a retrofitted design that was operated for two 1-month runs, achieving steady state with good material balance closure. Run 1 with the retrofitted reactor produced 50 g/L ethanol at a cellulose conversion of 74%. Run 2 produced 42 g/L ethanol at a conversion of 92%. For run 2, the ethanol yield was 0.466 g ethanol/g glucose equivalent fermented and >94% of the xylan fed to the reactor was solubilized to a mixture of xylan oligomers and xylose.
机译:为了在接近工业过程预期的条件下操作,研究了纸污泥向乙醇的转化。研究的漂白牛皮纸污泥的主要成分是葡聚糖(62 wt。%,干基),木聚糖(11.5%)和矿物质(17%)。在成分分析过程中要完全回收葡萄糖,需要进行两次酸水解处理,而不是一次。为避免混合未反应的造纸污泥的困难,开发了一种半连续固体进料的实验室生物反应器系统。该系统的特点是每隔12小时进料一次,停留时间为4天,纤维素酶负载量为15至20 FPU / g纤维素。使用同时补充糖化和发酵(SSF)的污泥转化为乙醇,其特征是补充了葡糖苷酶的商业纤维素酶制剂,并通过酿酒酵母进行了葡萄糖发酵。 SSF在第一代系统中进行了4个月,平均乙醇浓度为35 g / L。但是,没有达到稳定状态,并且遇到了操作困难。在经过两次为期1个月的运行改造的设计中避免了这些困难,从而实现了稳定状态并具有良好的物料平衡封闭性。使用改造后的反应器进行的运行1产生了50 g / L的乙醇,纤维素转化率为74%。试验2产生了42g / L乙醇,转化率为92%。对于运行2,乙醇产率为发酵的0.466g乙醇/ g葡萄糖当量,并且将进料至反应器的> 94%的木聚糖增溶为木聚糖低聚物和木糖的混合物。

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