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Insight into Pseudomonas aeruginosa pyocyanin production under low-shear modeled microgravity

机译：低重力模拟微重力下铜绿假单胞菌绿脓素生成的洞察

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摘要

Long-term space flight impairs the immune system of astronauts, rendering them vulnerable to opportunistic infections. Pseudomonas aeruginosa causes opportunistic infections, particularly in individuals with a compromised immune system; it can be a major health hazard for astronauts during space flight missions. Hence, the production of the most abundant redox active virulence factor, pyocyanin by P. aeruginosa, was assessed under low-shear modeled microgravity (LSMMG) conditions, simulated using a high aspect ratio vessel. Moreover, we evaluated changes in the expression of genes involved in pyocyanin biosynthesis and genes involved in the MexGHI-OpmD operon quorum sensing. Extracellular DNA and H2O2 production were measured, and their correlation with pyocyanin production was examined. Interestingly, the pyocyanin quantity was 2.58-fold lower in the LSMMG conditions compared to the normal gravity. LSMMG caused downregulation of the genes associated with pyocyanin biosynthesis. Interestingly, extracellular DNA and H2O2 release were significantly high in the normal gravity environment. Scanning electron microscopy revealed aggregation and elongated cells under LSMMG. Taken together, these findings suggest that LSMMG did not induce pyocyanin secretion in P. aeruginosa.
机译：长期的太空飞行会损害宇航员的免疫系统，使他们容易受到机会性感染。铜绿假单胞菌引起机会性感染，特别是在免疫系统受损的个体中；对于太空飞行任务的宇航员来说，这可能是主要的健康危害。因此，在低剪切模型微重力（LSMMG）条件下（使用高纵横比容器模拟）评估了铜绿假单胞菌产生的最丰富的氧化还原活性毒力因子绿脓素。此外，我们评估了参与花青素生物合成的基因和参与MexGHI-OpmD操纵子群体感应的基因的表达变化。测量了细胞外DNA和H2O2的产生，并检查了它们与绿脓素产生的相关性。有趣的是，在LSMMG条件下，与正常重力相比，绿脓素的含量低2.58倍。 LSMMG引起了与花青素生物合成有关的基因的下调。有趣的是，在正常重力环境下，细胞外DNA和H2O2的释放量很高。扫描电子显微镜显示在LSMGG下聚集和伸长的细胞。综上所述，这些发现表明LSMGG没有诱导铜绿假单胞菌中的绿脓素分泌。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2019年第2期|267-277|共11页
  • 作者

    Sheet Sunirmal; Sathishkumar Yesupatham; Choi Mi-Sook; Lee Yang Soo;

  • 作者单位

    Chonbuk Natl Univ, Coll Agr & Life Sci, Dept Forest Sci & Technol, 567 Baekje Daero, Jeonju Si, Jeollabuk Do, South Korea;

    Chonbuk Natl Univ, Coll Agr & Life Sci, Dept Forest Sci & Technol, 567 Baekje Daero, Jeonju Si, Jeollabuk Do, South Korea|Korea Adv Inst Sci & Technol, Dept Biol Sci, Daejeon, South Korea;

    Chonbuk Natl Univ, Coll Agr & Life Sci, Dept Forest Sci & Technol, 567 Baekje Daero, Jeonju Si, Jeollabuk Do, South Korea;

    Chonbuk Natl Univ, Coll Agr & Life Sci, Dept Forest Sci & Technol, 567 Baekje Daero, Jeonju Si, Jeollabuk Do, South Korea;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Low-shear modeled microgravity; Pyocyanin; Pseudomonas aeruginosa;

    机译：低剪切模拟微重力;花青素;铜绿假单胞菌;

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