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首页> 外文期刊>Bioprocess and Biosystems Engineering >Biosynthesis and accumulation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-polyethylene glycol, a hybrid co-polymer by endophytic Bacillus cereus RCL 02
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Biosynthesis and accumulation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-polyethylene glycol, a hybrid co-polymer by endophytic Bacillus cereus RCL 02

机译:内生蜡状芽孢杆菌RCL 02对杂化共聚物聚(3-羟基丁酸酯-共3-羟基戊酸酯)-聚乙二醇的生物合成和积累

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摘要

Co-polymerization of microbial polyesters, polyhydroxyalkanoates (PHAs), with synthetic polymers has become an established and promising tool in the recent past for improving the material and biological properties of the biopolyesters. Bacillus cereus RCL 02, a leaf endophytic bacterium of the oleaginous plant Ricinus communis L., has been reported to produce a significant amount of poly(3-hydroxybutyrate) [P(3HB)] and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] under batch cultivation. The present study demonstrates the synthesis and accumulation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-polyethylene glycol [P(3HB-co-3HV)-PEG] co-polymer by the isolate RCL 02 in glucose, valeric acid, and PEG-200 supplemented mineral salts medium following dual-step cultivation. The identity of P(3HB-co-3HV)-PEG co-polymer so produced has been confirmed by X-ray diffraction (XRD) analysis, Fourier-transform infrared (FTIR), and proton nuclear magnetic resonance (H-1 NMR) spectroscopic studies, and the purified co-polymer was found to be composed of 3.2mol% ethylene glycol (EG) and 8.4mol% 3HV along with 3HB. While the thermogravimetric analysis (TGA) revealed that P(3HB-co-3HV)-PEG films degraded at 269.32 degrees C, differential scanning calorimetry (DSC) recorded the melting peak of the co-polymer at 163.8 degrees C. This study emphasized to explore the endophytic Bacillus spp. for production of P(3HB-co-3HV)-PEG co-polymers with improved material properties which may find possible application for biomedical purposes.
机译:微生物聚酯,聚羟基链烷酸酯(PHA)与合成聚合物的共聚反应在最近几年已成为一种建立和有前途的工具,用于改善生物聚酯的材料和生物学特性。据报道,蜡状芽胞杆菌RCL 02是油质植物蓖麻(Ricinus communis L.)的叶内生细菌,可产生大量的聚3-羟基丁酸酯[P(3HB)]和聚3-羟基丁酸酯-co-3-分批培养中的[羟基戊酸酯] [P(3HB-co-3HV)]。本研究证明了分离物RCL 02在葡萄糖,戊酸中合成和积累聚(3-羟基丁酸酯-co-3-羟基戊酸酯)-聚乙二醇[P(3HB-co-3HV)-PEG]共聚物,两步培养后,添加PEG-200补充的无机盐培养基。通过X射线衍射(XRD)分析,傅立叶变换红外(FTIR)和质子核磁共振(H-1 NMR)证实了如此生产的P(3HB-co-3HV)-PEG共聚物的身份光谱研究,发现纯化的共聚物由3.2摩尔%乙二醇(EG)和8.4摩尔%3HV和3HB组成。热重分析(TGA)显示P(3HB-co-3HV)-PEG膜在269.32摄氏度下降解,而差示扫描量热法(DSC)记录了共聚物在163.8摄氏度下的熔融峰。探索内生芽孢杆菌属。用于生产具有改善的材料性能的P(3HB-co-3HV)-PEG共聚物,可能会发现其在生物医学方面的应用。

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