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Production of cutinase by solid-state fermentation and its use as adjuvant in bioherbicide formulation

机译:固态发酵生产角质酶及其在生物除草剂配方中的佐剂用途

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摘要

In the present study, it was presented a strategy to maximize the cutinase production by solid-state fermentation from different microorganisms and substrates. The best results were observed using Fusarium verticillioides, rice bran being the main substrate. Maximum yield of cutinase obtained by the strain was 16.22U/g. For concentration, ethanol precipitation was used, and the purification factor was 2.4. The optimum temperature and pH for enzyme activity were 35 degrees C and 6.5, respectively. The enzyme was stable at a wide range of temperature and at all pH values tested. The concentrated cutinase was used as an adjuvant in a formulation containing cutinase+bioherbicide. The use of enzyme increased the efficiency of bioherbicide, since cutinase was responsible to remove/degrade the cutin that recovery the weed leaves and difficult the bioherbicide absorption. Cutinase showed to be a promising product to be used in formulation of bioherbicides.
机译:在本研究中,提出了一种策略,可通过固态发酵从不同的微生物和底物中最大化角质酶的产生。使用米糠镰刀菌(Fusarium v​​erticillioides)为主要基质,观察到了最佳结果。通过该菌株获得的角质酶的最大产量为16.22U / g。为了浓缩,使用乙醇沉淀,并且纯化因子为2.4。酶活性的最佳温度和pH分别为35摄氏度和6.5。该酶在很宽的温度范围和所有测试的pH值下均稳定。浓缩的角质酶在含有角质酶+生物除草剂的制剂中用作佐剂。酶的使用提高了生物除草剂的效率,因为角质酶负责去除/降解角质,从而回收杂草叶并难以吸收生物除草剂。角质酶显示是用于生物除草剂配方的有前途的产品。

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