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首页> 外文期刊>Bioprocess and Biosystems Engineering >Characterization of pyrene and chrysene degradation by halophilic Hortaea sp. B15
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Characterization of pyrene and chrysene degradation by halophilic Hortaea sp. B15

机译：嗜盐Hortaea sp。的of和ch降解的表征。 B15

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摘要

Polycyclic aromatics hydrocarbons (PAHs) are ubiquitous and toxic pollutants that are dangerous to humans and living organism in aquatic environment. Normally, PAHs has lower molecular weight such as phenanthrene and naphthalene that are easy and efficient to degrade, but high-molecular-weight PAHs such as chrysene and pyrene are difficult to be biodegraded by common microorganism. This study investigated the isolation and characterization of a potential halophilic bacterium capable of utilizing two high-molecular-weight PAHs. At the end of the experiment (25-30days of incubation), bacterial counts have reached a maximum level (over 40x10(16)CFU/mL). The highest biodegradation rate of 77% of chrysene in 20days and 92% of pyrene in 25days was obtained at pH 7, temperature 25 degrees C, agitation of 150rpm and Tween 80 surfactant showing to be the most impressive parameters for HMWPAHs biodegradation in this research. The metabolism of initial compounds revealed that Hortaea sp. B15 utilized pyrene to form phthalic acid while chrysene was metabolized to form 1-hydroxy-2-naphthoic acid. The result showed that Hortaea sp. B15 can be promoted for the study of in situ biodegradation of high molecular weight PAH.
机译：多环芳烃（PAHs）是普遍存在的有毒污染物，对水生环境中的人类和生物具有危险。通常，PAH具有较低的分子量，例如菲和萘，易于降解和降解，但是高分子量的PAHs（例如，and和and）很难被常见的微生物生物降解。这项研究调查了能够利用两个高分子量PAHs的潜在嗜盐细菌的分离和表征。在实验结束时（孵育25-30天），细菌计数已达到最高水平（超过40x10（16）CFU / mL）。在pH值为7，温度为25摄氏度，搅拌速度为150rpm和吐温80表面活性剂的条件下，在20天内的77％的and和25天内的92％的bio具有最高的生物降解率，这是该研究中HMWPAHs生物降解的最令人印象深刻的参数。初始化合物的代谢显示Hortaea sp。 B15利用pyr形成邻苯二甲酸，而新陈代谢则形成1-羟基-2-萘甲酸。结果显示Hortaea sp.。可以促进B15用于高分子量PAH的原位生物降解研究。

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