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首页> 外文期刊>Bioprocess and Biosystems Engineering >Effect of aeration and agitation on yeast inulinase production: a biocalorimetric investigation
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Effect of aeration and agitation on yeast inulinase production: a biocalorimetric investigation

机译：曝气与搅拌对酵母菊粉酶生产的影响：生物植物调查

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摘要

Air flow rate and agitation speed for inulinase production by Kluyveromyces marxianus were optimized based on metabolic heat release profiles. Shear stress and oxygen transfer (k(L)a) values were compared to assess the effects of aeration and agitation. At agitation rates of 100rpm, the oxygen mass transfer rates were small and eventually led to less inulinase production, but at agitation rates 150rpm, loss of biomass resulted in less inulinase activity. Bio-reaction calorimeter (BioRc1e) experiment with aeration rates0.5 lpm showed low k(L)a while at 1.5 lpm frothing of reactor contents caused loss of biomass and inulinase activity. The optimum conditions for aeration and agitation rate for K. marxianus in BioRc1e were 1 lpm and 150rpm. Heat yield values obtained for the substrate, product and biomass reinstated the ongoing metabolic process. The heat release pattern could be a promising tool for optimization of bioprocess and in situ monitoring, with a possibility of interventions during the biotransformation process. At optimized aeration and agitation conditions, a two-fold increase in inulinase activity could be noticed.[GRAPHICS].
机译：基于代谢热释放型材优化了Kluyveromyces Marxianus的空气流量和胰岛素生产搅拌速度。比较剪切应力和氧气转移（K（L）A）值，以评估通气和搅拌的影响。在100rpm的搅拌速率下，氧气传质率小，最终导致含硫酸盐酶的产生较少，但在搅拌速率> 150rpm时，生物质的损失导致含硫酸盐酶活性较少。曝气率的生物反应量热计（BiORC1E）实验率为0.5LPM显示低K（L）A虽然在1.5Lpm的反应器内容物中引起生物质和氨基蛋白酶活性的损失。 K.Miorc1e中K.Marxianus的曝气和搅拌速率的最佳条件为1磅和150rpm。为基材，产品和生物质获得的热产率值恢复了正在进行的代谢过程。热释放图案可以是用于优化生物过程和原位监测的有希望的工具，其在生物转化过程中的干预措施可能性。在优化的曝气和搅拌条件下，可以注意到菊粉激酶活性的两倍增加。[图形]。

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