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首页> 外文期刊>Bioprocess and Biosystems Engineering >Enhanced tolerance to inhibitors of Escherichia coli by heterologous expression of cyclopropane-fatty acid-acyl-phospholipid synthase (cfa) from Halomonas soda
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Enhanced tolerance to inhibitors of Escherichia coli by heterologous expression of cyclopropane-fatty acid-acyl-phospholipid synthase (cfa) from Halomonas soda

机译：通过来自Halomonas苏打水的环丙烷 - 脂肪酸 - 酰氯 - 磷脂合酶（CFA）的异源表达来增强对大肠杆菌抑制剂的耐受性

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摘要

Bacteria have evolved a defense system to resist external stressors, such as heat, pH, and salt, so as to facilitate survival in changing or harsh environments. However, the specific mechanisms by which bacteria respond to such environmental changes are not completely elucidated. Here, we used halotolerant bacteria as a model to understand the mechanism conferring high tolerance to NaCl. We screened for genes related to halotolerance in Halomonas socia, which can provide guidance for practical application. Phospholipid fatty acid analysis showed that H. socia cultured under high osmotic pressure produced a high portion of cyclopropane fatty acid derivatives, encoded by the cyclopropane-fatty acid-acyl phospholipid synthase gene (cfa). Therefore, H. socia cfa was cloned and introduced into Escherichia coli for expression. The cfa-overexpressing E. coli strain showed better growth, compared with the control strain under normal cultivation condition as well as under osmotic pressure ( 3% salinity). Moreover, the cfa-overexpressing E. coli strain showed 1.58-, 1.78-, 3.3-, and 2.19-fold higher growth than the control strain in the presence of the inhibitors furfural, 4-hydroxybenzaldehyde, vanillin, and acetate from lignocellulosic biomass pretreatment, respectively. From a practical application perspective, cfa was co-expressed in E. coli with the polyhydroxyalkanoate (PHA) synthetic operon of Ralstonia eutropha using synthetic and biosugar media, resulting in a 1.5-fold higher in PHA production than that of the control strain. Overall, this study demonstrates the potential of the cfa gene to boost cell growth and production even in heterologous strains under stress conditions.
机译：细菌已经进化了防御系统来抵抗外部压力，如热，pH和盐，以便于改变或恶劣环境中的生存。然而，细菌对这种环境变化的响应的特定机制并不完全阐明。在这里，我们使用Halotolerant细菌作为模型，以了解赋予NaCl高耐受性的机制。我们筛查了与HaloMonas Socia的Halotolerance相关的基因，可以为实际应用提供指导。磷脂脂肪酸分析表明，在高渗透压下培养的H.SoCia产生了一部分的环丙烷脂肪酸衍生物，由环丙烷 - 脂肪酸 - 酰基磷脂合酶基因（CFA）编码。因此，H.Socia CFA被克隆并引入大肠杆菌中的表达。 CFA过表达大肠杆菌菌株表现出更好的生长，与正常培养条件下的对照菌株以及渗透压（> 3％盐度）相比。此外，CFA过表达大肠杆菌菌株显示出1.58-，1.78-，3.3-和2.19倍的生长，而不是木质纤维素生物质预处理的抑制剂糠醛，4-羟基苯甲醛，香草素和醋酸盐的对照菌株， 分别。从实际应用的角度来看，使用合成和生物糖培养基，CFA与Ralstonia Eutropha的聚羟基烷酸盐（PHA）合成操纵子共同表达，从而在PHA产生中比对照菌株的高1.5倍。总体而言，该研究表明CFA基因的潜力即使在胁迫条件下的异源菌株中也能够提高细胞生长和生产。

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