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首页> 外文期刊>Bioprocess and Biosystems Engineering >Investigation of the reversibility of freeze/thaw stress-induced protein instability using heat cycling as a function of different cryoprotectants
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Investigation of the reversibility of freeze/thaw stress-induced protein instability using heat cycling as a function of different cryoprotectants

机译：使用热循环作为不同冷冻保护剂的函数的冻融/解冻应激诱导蛋白质不稳定性的调查

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Formulation conditions have a significant influence on the degree of freeze/thaw (FT) stress-induced protein instabilities. Adding cryoprotectants might stabilize the induced FT stress instabilities. However, a simple preservation of protein stability might be insufficient and further methods are necessary. This study aims to evaluate the addition of a heat cycle following FT application as a function of different cryoprotectants with lysozyme as exemplary protein. Sucrose and glycerol were shown to be the most effective cryoprotectants when compared to PEG200 and Tween20. In terms of heat-induced reversibility of aggregates, glycerol showed the best performance followed by sucrose, NaCl and Tween20 systems. The analysis was performed using a novel approach to visualize complex interplays by a clustering and data reduction scheme. In addition, solubility and structural integrity were measured and confirmed the obtained results.[GRAPHICS].
机译：配方条件对冷冻/解冻（FT）应激诱导的蛋白质不稳定性具有显着影响。添加冷冻保护剂可能会稳定诱导的FT压力稳定性。然而，可以简单的蛋白质稳定性保存可能不足，并且需要进一步的方法。本研究旨在根据用溶菌酶作为示例性蛋白质的不同冷冻保护剂的函数评估加热循环的加热。与PEG200和TWEEN20相比，蔗糖和甘油显示为最有效的冷冻保护剂。就聚集体的热诱导可逆性而言，甘油显示出最佳性能，然后是蔗糖，NaCl和Tween20系统。使用一种新的方法来进行分析来通过聚类和数据减少方案来可视化复杂相互作用。此外，测量溶解度和结构完整性并确认得到的结果。[图形]。

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