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首页> 外文期刊>Bioprocess and Biosystems Engineering >Microbial lipid production by oleaginous yeasts grown on Scenedesmus obtusiusculus microalgae biomass hydrolysate
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Microbial lipid production by oleaginous yeasts grown on Scenedesmus obtusiusculus microalgae biomass hydrolysate

机译：在Scenedesmus oldusiusculus微藻生物量水解产物上生长的微生物脂质生产

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摘要

Due to increasing oil prices and climate change concerns, biofuels have become increasingly important as potential alternative energy sources. However, the use of arable lands and valuable resources for the production of biofuel feedstock compromises food security and negatively affect the environment. Single cell oils (SCOs), accumulated by oleaginous yeasts, show great promise for efficient production of biofuels. However, the high production costs attributed to feedstocks or raw materials present a major limiting factor. The fermentative conversion of abundant, low-value biomass into microbial oil would alleviate this limitation. Here, we explore the feasibility of utilizing microalgae-based cell residues as feedstock for yeast oil production. We developed an efficient, single-step enzymatic hydrolysis to generate Scenedesmus obtusiusculus hydrolysate (SH) without thermo-chemical pretreatment. With this eco-friendly process, glucose conversion efficiencies reached 90-100%. Cutaneotrichosporon oleaginosus, Cryptococcus curvatus and Rhodosporidium toruloides were cultivated on SH as sole nutrients source. Only C. oleaginosus was able to accumulate intracellular lipids, with a 35% (g lipid/g DCW) content and a yield of 3.6 g/L. Our results demonstrate the potential valorization of algal biomass into desired end-products such as biofuels. Graphic
机译：由于油价增加和气候变化的关注，生物燃料已成为潜在的替代能源越来越重要。然而，使用耕地和宝贵资源为生物燃料原料的生产损害了粮食安全，对环境产生负面影响。由含油酵母累积的单细胞油（SCOS），对生物燃料的有效生产具有很大的承诺。然而，归因于原料或原料的高生产成本存在主要限制因素。丰富，低值生物质的发酵转化为微生物油将减轻这种限制。在这里，我们探讨利用基于微藻的细胞残留作为酵母油生产原料的可行性。我们开发了一种有效的单步酶水解，以产生肌腱分离酶（SH）而无需热化学预处理。凭借这种环保过程，葡萄糖转化效率达到90-100％。 Cutaneotrichosporon oferealosus，Cryptococcus curvatus和rhodosporidium toruloides在sh中培养，作为唯一的营养素来源。只有C. oleaginosus能够积聚细胞内脂质，35％（g脂质/ g dcw）含量和3.6g / l的产率。我们的结果证明了藻类生物量的潜在算命，进入所需的最终产品，例如生物燃料。形象的

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