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首页> 外文期刊>Bioprocess and Biosystems Engineering >Directed evolution of Aspergillus oryzae lipase for the efficient resolution of (R,S)-ethyl-2-(4-hydroxyphenoxy) propanoate
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Directed evolution of Aspergillus oryzae lipase for the efficient resolution of (R,S)-ethyl-2-(4-hydroxyphenoxy) propanoate

机译：曲霉菌脂肪酶的定向演变，为（R，S） - 乙基-2-（4-羟基氧基）丙酸乙酯的有效分离

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摘要

Aspergillus oryzaelipase (AOL) is a potential biocatalyst for industrial application. In this study, a mutant lipase AOL-3(F38N/V230R)was screened through two rounds of directed evolution, resulting in a fourfold increase in lipase activity, and threefold in catalytic efficiency (k(cat)/K-m), while maintaining its excellent stereoselectivity. AOL-3(F38N/V230R)enzyme activity was maximum at pH 7.5 and also at 40 degrees C. And compared with wild-type AOL-3, AOL-3(F38N/V230R)preferentially hydrolyzed the fatty acid ethyl ester carbon chain length from C4 to C6-C10. In the same catalytic reaction conditions, the conversion of (R,S)-ethyl-2-(4-hydroxyphenoxy) propanoate ((R,S)-EHPP) by AOL-3(F38N/V230R)can be increased 169.7% compared to the original enzyme. The e.e.(s)of (R,S)-EHPP achieved 99.4% and conversion about 50.2% with E value being 829.0. Therefore, AOL-3(F38N/V230R)was a potential biocatalyst for obtaining key chiral compounds for aryloxyphenoxy propionate (APP) herbicides.
机译：Aspergillus Oryzaelipase（AOL）是工业应用的潜在生物催化剂。在该研究中，通过两轮定向的进化筛选突变脂肪酶AOL-3（F38N / V230R），导致脂肪酶活性的四倍增加，催化效率的三倍（K（猫）/ Km），同时保持其优异的立体选择性。 AOL-3（F38N / V230R）酶活性在pH7.5时最大，也是40℃并与野生型AOL-3相比，AOL-3（F38N / V230R）优先水解脂肪酸乙酯碳链长度从C4到C6-C10。在相同的催化反应条件下，通过AOL-3（F38N / V230R）的（R，S） - 乙基-2-（4-羟基苯氧基）丙酸乙酯（（R，S）-EHPP）的转化率可以增加169.7％对原始酶。 （R，S）-ehpp的例如（r，s）达到99.4％，转化为约50.2％，E值为829.0。因此，AOL-3（F38N / V230R）是潜在的生物催化剂，用于获得芳氧基苯氧基丙酸酯（APP）除草剂的关键手性化合物。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2020年第12期|2131-2141|共11页
  • 作者

    Zhang Mengjie; Li Qi; Lan Xing; Li Xiaojun; Zhang Yinjun; Wang Zhao; Zheng Jianyong;

  • 作者单位

    Zhejiang Univ Technol Coll Biotechnol & Bioengn Key Lab Bioorgan Synth Zhejiang Prov Hangzhou 310014 Zhejiang Peoples R China;

    Zhejiang Univ Technol Coll Biotechnol & Bioengn Key Lab Bioorgan Synth Zhejiang Prov Hangzhou 310014 Zhejiang Peoples R China;

    Zhejiang Univ Technol Coll Biotechnol & Bioengn Key Lab Bioorgan Synth Zhejiang Prov Hangzhou 310014 Zhejiang Peoples R China;

    Xinyu Univ Sch Med & Life Sci Xinyu Jiangxi Peoples R China;

    Zhejiang Univ Technol Coll Biotechnol & Bioengn Key Lab Bioorgan Synth Zhejiang Prov Hangzhou 310014 Zhejiang Peoples R China;

    Zhejiang Univ Technol Coll Biotechnol & Bioengn Key Lab Bioorgan Synth Zhejiang Prov Hangzhou 310014 Zhejiang Peoples R China;

    Zhejiang Univ Technol Coll Biotechnol & Bioengn Key Lab Bioorgan Synth Zhejiang Prov Hangzhou 310014 Zhejiang Peoples R China;

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  • 正文语种 eng
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  • 关键词

    Aspergillus oryzaelipase; Directed evolution; Enzymatic characterization; Molecular docking; Biocatalysis;

    机译：Aspergillus oryzaelipase;定向演变;酶促表征;分子对接;生物催化;

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