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首页> 外文期刊>Bioprocess and Biosystems Engineering >Valorization of apple pomace using bio-based technology for the production of xylitol and 2G ethanol
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Valorization of apple pomace using bio-based technology for the production of xylitol and 2G ethanol

机译：苹果渣利用基于生物的技术为木糖醇和2G乙醇生产的苹果渣技术

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Apple pomace was studied as a raw material for the production of xylitol and 2G ethanol, since this agroindustrial residue has a high concentration of carbohydrate macromolecules, but is still poorly studied for the production of fermentation bioproducts, such as polyols. The dry biomass was subjected to dilute-acid hydrolysis with H(2)SO(4)to obtain the hemicellulosic hydrolysate, which was concentrated, detoxified and fermented. The hydrolyzate after characterization was submitted to submerged fermentations, which were carried out in Erlenmeyer flasks using, separately, the yeastsCandida guilliermondiiandKluyveromyces marxianus. High cellulose (32.62%) and hemicellulose (23.60%) contents were found in this biomass, and the chemical hydrolysis yielded appreciable quantities of fermentable sugars, especially xylose. Both yeasts were able to metabolize xylose, butCandida guilliermondiiproduced only xylitol (9.35 g L(-1)in 96 h), whileK.marxianusproduced ethanol as the main product (10.47 g L(-1)in 24 h) and xylitol as byproduct (9.10 g L(-1)xylitol in 96 h). Maximum activities of xylose reductase and xylitol dehydrogenase were verified after 24 h of fermentation withC.guilliermondii(0.23 and 0.53 U/mg(prot), respectively) and withK.marxianus(0.08 e 0.08 U/mg(prot), respectively). Apple pomace has shown potential as a raw material for the fermentation process, and the development of a biotechnological platform for the integrated use of both the hemicellulosic and cellulosic fraction could add value to this residue and the apple production chain.
机译：苹果渣作为制备木糖醇和2g乙醇的原料，因为该农业工业残留物具有高浓度的碳水化合物大分子，但仍然对发酵生物制作的生产差异很差，例如多元醇。用H（2）如（4）进行干燥的生物质稀释酸水解，得到半纤维素水解产物，浓缩，疏散和发酵。表征后的水解产物被提交给浸没式发酵，其在Erlenmeyer烧瓶中使用，单独使用，酵母菌菌菌酵母烧伤的烧伤症进行。在该生物质中发现高纤维素（32.62％）和半纤维素（23.60％）含量，化学水解产生了明显的可发酵糖，特别是木糖。两种酵母都能够代谢木糖，Butcandida guilliermondiiproduced仅在96小时内（9.35g L（-1）），而kianualusproducy乙醇作为主要产物（10.47g l（-1）24小时）和木糖醇作为副产物（ 9.10g l（-1）96 h中的木糖醇。在24小时后24小时后验证了木糖还原酶和木糖醇脱氢酶的最大活性（分别为0.23和0.53 U / mg（prot））和用k.marxianus（0.08 e 0.08 U / mg（prot））。 Apple Pomace表现为发酵过程的原料，并且生物技术平台的开发用于综合使用半纤维素和纤维素级分，可以为该残留物和苹果生产链增加价值。

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