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首页> 外文期刊>Bioprocess and Biosystems Engineering >Proteomic analysis and optimized production of Alkalihalobacillus patagoniensis PAT 05~T extracellular proteases
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Proteomic analysis and optimized production of Alkalihalobacillus patagoniensis PAT 05~T extracellular proteases

机译：蛋白质组学分析和优化生产碱卤代曲杆菌PATOGANISIS PAT 05〜T细胞外蛋白酶

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摘要

Extracellular proteolytic extracts from the haloalkalitolerant strainAlkalihalobacillus patagoniensisPAT 05(T) have proved highly efficient to reduce wool felting, as part of an ecofriendly treatment suitable for organic wool. In the present study, we identified the extracellular proteases produced by PAT 05(T) and we optimized its growth conditions for protease production through statistical methods. A total of 191 proteins were identified in PAT 05(T) culture supernatants through mass spectrometry analysis. Three of the 6 detected extracellular proteases belonged to the serine-endopeptidase family S8 (EC 3.4.21); two of them showed 86.3 and 67.9% identity with an alkaline protease fromBacillus alcalophilusand another one showed 50.4% identity with Bacillopeptidase F. The other 3 proteases exhibited 55.3, 49.4 and 61.1% identity with D-alanyl-D-alanine carboxypeptidase DacF, D-alanyl-D-alanine carboxypeptidase DacC and endopeptidase LytE, respectively. Using a Fractional Factorial Design followed by a Central Composite Design optimization, a twofold increase in protease production was reached. NaCl concentration was the most influential factor on protease production. The usefulness of PAT 05(T) extracellular proteolytic extracts to reduce wool felting was possible associated with the activity of the serine-endopeptidases closely related to highly alkaline keratinolytic proteases. The other identified proteases could cooperate, improving protein hydrolysis. This study provided valuable information for the exploitation of PAT 05(T) proteases which have potential for the valorization of organic wool as well as for other industrial applications.[GRAPHICS].
机译：来自Haloalkalitolerentantantantantstallihalobacillus patagoniensispat 05（t）的细胞外蛋白水解提取物已经证明了减少羊毛毡的高效，作为适用于有机羊毛的Ecofriendly治疗的一部分。在本研究中，我们鉴定了PAT 05（T）产生的细胞外蛋白酶，我们通过统计方法优化了蛋白酶产生的生长条件。通过质谱分析在Pat 05（T）培养上清液中共鉴定了总共191种蛋白质。 6个检测到的细胞外蛋白酶中有三种属于丝氨酸 - 内肽酶S8（EC 3.4.21）;其中两个呈86.3和67.9％的同一性，碱性蛋白酶从碧眼的胰岛素起草，另一个蛋白酶含有50.4％的同一性与枯草骨肽酶F.另外3个蛋白酶呈现55.3,49.4和61.1％同一性与D- alanyl-D-丙氨酸羧肽酶Dacf，D- Alanyl-D-丙氨酸羧肽酶DACC和内肽酶Lyte。使用分数阶乘设计之后进行中央复合设计优化，达到了两种蛋白酶生产的增加。 NaCl浓度是蛋白酶生产最有影响力的因素。 PAT 05（T）细胞外蛋白水解提取物减少羊毛纤维的有用性是可能与高碱性角质溶解蛋白酶密切相关的丝氨酸内肽酶的活性相关的。其他鉴定的蛋白酶可以合作，改善蛋白质水解。本研究提供了有价值的信息，用于剥削PAT 05（T）蛋白酶，该蛋白酶具有有机羊毛的储价以及其他工业应用的潜力。[图形]。

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