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首页> 外文期刊>Bioprocess and Biosystems Engineering >Key parameters optimization of chitosan production from Aspergillus terreus using apple waste extract as sole carbon source
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Key parameters optimization of chitosan production from Aspergillus terreus using apple waste extract as sole carbon source

机译：用苹果废物提取物作为含苹果废物提取物作为唯一碳源的壳聚糖生产的关键参数优化

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摘要

Chitosan is commonly obtained from shrimp and crab shell chitin by deacetylation; however, such supplies appear limitation. An alternative source of chitosan is cell wall in certain fungi. In this study, chitosan production through submerged fermentation ofAspergillus terreuson apple waste extract as sole carbon source was investigated. Monod equation with a maximum specific growth rate of 0.083 h(-1)and half-saturation constant of 6.67 w/v% was best described the kinetic of growth. Results of response surface methodology showed the highest chitosan to substrate yield of 49.32 mg g(substrate)(-1), chitosan to fungal biomass yield of 140.9 mg g(cell)(-1), and fungal biomass to substrate yield of 0.387 g(cell)g(substrate)(-1)were simultaneously obtained at temperature 30.0 degrees C, initial pH 5.98, and ammonium nitrate concentration 5.0 g L-1. The chitosan produced at the optimum condition was characterized by FTIR, TGA, and DSC analysis, and degree of deacetylation was 88.2%.[GRAPHICS].
机译：壳聚糖通常通过脱乙酰化从虾和蟹壳依甲酸盐中获得;但是，这种用品看起来有限。壳聚糖的替代来源是某些真菌的细胞壁。在本研究中，研究了壳聚糖通过淹没的发酵作为唯一的碳源淹没发酵作为唯一碳源。最大特异性生长速率为0.083h（-1）和半饱和度常数为6.67w / v％的单架等式最多描述了生长的动力学。响应表面方法的结果显示出49.32mg g（底物）（ - 1），壳聚糖对140.9mg g（细胞）（ - 1）的真菌生物质产率为40.9mg（ - 1）的基质产率，对底物产率为0.387g （细胞）g（衬底）（ - 1）在温度30.0℃，初始pH 5.98和硝酸铵浓度5.0g L-1的温度下同时获得。在最佳条件下产生的壳聚糖的特征在于FTIR，TGA和DSC分析，脱乙酰化程度为88.2％。[图形]。

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