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Biohydrogen production in an AFBR using sugarcane molasses

机译:使用甘蔗糖蜜的AFBR中的生物氢生产

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This study evaluated an anaerobic fluidized bed reactor to produce hydrogen from sugarcane molasses of 25 g-COD L-1. The reactor of 1.2 L working volume contained shredded tires as support material. The inoculum was sludge obtained in a UASB reactor of a sewage treatment plant. The AFBR was operated at hydraulic retention times of 12, 6, 4 and 3 h. The maximum hydrogen production rate (1.44 L-H-2 h(-1) L-1) and the highest hydrogen yield (3.07 mol-H-2 mol(-1)-glucose) occurred at HRT of 4 and 6 h, respectively. The highest COD removal (23.3 +/- 8.5%) was achieved at HRT of 12 h, while the HRT of 6 h presented the maximum carbohydrate conversion of 70.1 +/- 2.2%. Ethanol (44-67%) and acetic acid (18-38%) were the main metabolites produced, emphasizing a predominance of ethanol-type fermentation pathway in the process. The PCR-DGGE analysis revealed that the bacterial community presented a maximum similarity of 88% between HRT of 4 and 3 h, indicating that the microbial dynamic altered as the organic load has increased. The highest Shannon-Winner index of 2.77 was obtained at HRT of 6 h, inferring that higher microbial diversity favored hydrogen production.
机译:该研究评估了厌氧流化床反应器,以产生25g-COD L-1的甘蔗糖蜜的氢。 1.2 L工作体积的反应器含有碎轮胎作为支撑材料。接种物是在污水处理厂的UASB反应器中获得的污泥。 AFBR在12,6,4和3小时的液压保留次数下操作。最大氢气生产速率(1.44 L-H-2 H(-1)L-1)和最高氢率(3.07mol-H-2mol(-1)葡糖)分别发生在4和6小时的HRT。在12小时的HRT中达到最高的鳕鱼(23.3 +/- 8.5%),而6小时的HRT呈现最大碳水化合物转化率为70.1 +/- 2.2%。乙醇(44-67%)和乙酸(18-38%)是主要代谢物,强调该过程中乙醇型发酵途径的优势。 PCR-DGGE分析显示,细菌群落在4和3小时之间呈现88%的最大相似性,表明随着有机载荷的增加而改变的微生物动态增加。 2.77的最高香农获奖指数在6小时的HRT获得,推断出更高的微生物多样性有利于氢气产生。

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