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首页> 外文期刊>Bioprocess and Biosystems Engineering >Bacterial community analysis of biofilm on API 5LX carbon steel in an oil reservoir environment
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Bacterial community analysis of biofilm on API 5LX carbon steel in an oil reservoir environment

机译：油藏环境中API 5LX碳钢的生物膜细菌群落分析

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摘要

This study aimed to characterize the biofilm microbial community that causes corrosion of API 5LX carbon steel. API 5LX carbon steel coupons were incubated with raw produced water collected from two oil reservoir stations or filter-sterilized produced water. Biofilm 16S rRNA amplicon sequencing revealed that the bacterial community present in the biofilm was dominated by Proteobacteria, includingMarinobacter hydrocarbonoclausticsandMarinobacter alkaliphilus. Electrochemical analysis such as impedance and polarization results indicated that Proteobacteria biofilm accelerated corrosion by similar to twofold (2.1 +/- 0.61 mm/years) or similar to fourfold (similar to 3.7 +/- 0.42 mm/years) when compared to the control treatment (0.95 +/- 0.1 mm/years). Scanning electron and atomic force microscopy revealed the presence of a thick biofilm and pitting corrosion. X-ray diffraction revealed higher amounts of the corrosion products Fe2O3, gamma-FeOOH, and alpha-FeOOH, and confirmed that the microbial biofilm strongly oxidized the iron and contributed to the acceleration of corrosion of carbon metal API 5LX.
机译：本研究旨在表征生物膜微生物群落，导致API 5LX碳钢的腐蚀。将API 5LX碳钢优惠券与从两个储油站收集的原料生产的水孵育，或滤过灭菌的产水。 Biofilm 16s RRNA扩增子测序显示，生物膜中存在的细菌群落由植物植物中的菌，包括嗜酸杆菌嗜含量分泌物和碱性碱性碱。电化学分析如阻抗和极化结果表明，与对照处理相比，促菌生物膜（2.1 +/- 0.61毫米/岁）与双重（类似于3.7 +/- 0.42毫米/岁）相比，生物膜生物膜促进腐蚀（0.95 +/- 0.1毫米/年）。扫描电子和原子力显微镜显示出厚生物膜的存在和蚀腐蚀。 X射线衍射显示较高量的腐蚀产物Fe2O3，γ-FeOOH和α-FeO​​HH，并证实微生物生物膜强烈氧化铁并导致碳金属API 5LX腐蚀的加速度。

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