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首页> 外文期刊>Bioprocess and Biosystems Engineering >Enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) biopolymer by recombinant Bacillus megaterium in fed-batch bioreactors
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Enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) biopolymer by recombinant Bacillus megaterium in fed-batch bioreactors

机译：通过在FED批量生物反应器中通过重组芽孢杆菌的聚（3-羟基丁酸酯-CO-3-羟基羟基戊烯）生物聚合物的生产增强

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Polyhydroxyalkanoates (PHAs) are biodegradable polyesters accumulated in a wide variety of microorganisms as intracellular carbon and energy storage compounds. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is one of the most valuable biopolymers because of its superior mechanical properties. Here, we developed a bioprocess utilizing recombinantBacillus megateriumstrain for PHBV over-production from glucose, without any precursor addition. PHA production was performed in a controlled bioreactor by batch and fed-batch modes using wild-typeB. megateriumand rec-B. megateriumcells overexpressing the nativephaCgene. The effect of oxygen transfer rate on biomass formation and PHA accumulation was also investigated, under different dissolved oxygen levels. Structural and thermal properties of PHA were characterized by GC-FID,H-1-NMR, TGA and DSC analyses. Significantly, the copolymer produced from glucose as the carbon source in rec-B. megateriumwas composed of 58 mol% of 3-hydroxyvalerate monomers. After 66 h, rec-B. megateriumcells in fed-batch fermentation with a pre-determined growth rate mu(0) = 0.1 h(-1)produced the highest CDW (7.7 g L-1) and PHA concentration (6.1 g L-1). Moreover, an exponential glucose feeding profile resulted in 2.2-fold increase in PHA yield compared to batch cultivation. Overall, this study paves the way to an enhanced biopolymer production process inB. megateriumcells, where the highest product yield on cell was obtained asY(P/X) = 0.8 g g(-1).
机译：多羟基烷烷（PHA）是可生物降解的聚酯，其在各种微生物中累积，作为细胞内碳和能量储存化合物。聚（3-羟基丁酸酯-CO-3-羟基戊戊烷）（PHBV）是其最有价值的生物聚合物之一，因为其优越的机械性能。在这里，我们开发了一种利用RefombinantBacillus MegaTiumStrain的生物处理，用于从葡萄糖中产生PHBV过度生产，而没有任何前体添加。通过使用野生型B通过批量和补料分批模式在受控生物反应器中进行PHA生产。 MegaTeriumand Rec-b。过表达海豚的MegaTiumcells。还研究了不同溶解氧水平的氧传输率对生物质形成和PHA积累的影响。通过GC-FID，H-1-NMR，TGA和DSC分析表征PHA的结构和热性质。值得注意的是，由葡萄糖产生的共聚物作为REC-B中的碳源。 MegaTiumwas由58摩尔％的3-羟基戊戊单体组成。 66小时后，REC-B。用预定的生长速率mu（0）= 0.1h（-1）产生最高CDW（7.7g L-1）和PHA浓度（6.1g L-1）的绵延的发酵中的MegaTiumcells。此外，与分批培养相比，指数葡萄糖喂养曲线导致PHA产率增加2.2倍。总的来说，这项研究铺平了增强的生物聚合物生产过程的方法。获得电池上最高产量的MegaTiumcells Asy（p / x）= 0.8g g（-1）。

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