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首页> 外文期刊>Bioprocess and Biosystems Engineering >A selected bacterial strain for the self-healing process in cementitious specimens without cell immobilization steps
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A selected bacterial strain for the self-healing process in cementitious specimens without cell immobilization steps

机译：用于无细胞固定步骤的胶凝样本中的自我愈合过程的选定细菌菌株

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The use of microorganisms capable of mediating the bioprecipitation process can be an important application in the self-healing processes of cement specimens. Thus, the present study identified and evaluated fiveBacillusstrains for potential application in the protocol of self-healing via bioprecipitation. Cell growth, enzyme production, and kinetic parameters conditions were evaluated during the fermentation process. Based on the analysis of 16S rDNA in conjunction with biochemical testing, results demonstrate that the strains are eitherBacillus cereusorBacillus thuringiensis. Strategically it was found that the addition of glycerol to fermentative medium was essential to increase the bacterial concentration (approximate to 4.2 x 10(7)cells mL(-1)) and production of the enzyme urease (approximate to 3.623,2 U.mL(-1)). The addition of this medium after 40 days of fermentation promoted the self-healing of cracks and increased compressive strength in approximate to 14.2% of the cementitious specimens; therefore, increasing the sustainability and engineering properties of cement-based materials.
机译：使用能够介导生物沉淀过程的微生物可以是水泥标本的自愈合过程中的重要应用。因此，本研究鉴定并评估了五十溴胞杆菌，用于通过生物沉淀的自我愈合方案中的潜在应用。在发酵过程中评估细胞生长，酶产生和动力学参数条件。基于16S RDNA的分析结合生化检测，结果表明，菌株是乌西苏氏菌属植物。策略性地发现，将甘油加入发酵培养基对于增加细菌浓度（近似至4.2×10（7）个细胞ml（-1））和酶脲酶的产生（近似为3.623,2甲基（-1））。在发酵40天后加入该培养基促进了裂缝的自我愈合，并增加了近似胶质标本的14.2％的抗压强度;因此，提高了基于水泥材料的可持续性和工程性能。

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