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首页> 外文期刊>Bioprocess and Biosystems Engineering >Enhancing ectoine production by recombinant Escherichia coli through step-wise fermentation optimization strategy based on kinetic analysis
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Enhancing ectoine production by recombinant Escherichia coli through step-wise fermentation optimization strategy based on kinetic analysis

机译：通过基于动力学分析的逐步发酵优化策略，通过重组大肠杆菌增强胞外产生的产量

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摘要

In this study, the recombinant ectoine-producing Escherichia coli ET01 was constructed by introducing the ectABC operon from Halomonas venusta ZH. To further improve ectoine production, the regulation of the fermentation process was systematically investigated. First, the effects of the initial glucose concentrations and glucose feeding mode on ectoine production were analyzed. Using a combination of pH-feedback feeding and glucose-controlled feeding, the ectoine titer reached 25.5 g/L, representing an 8.8-fold increase over standard batch culture. Then, the effects of dissolved oxygen (DO) levels (50, 40, 30, or 20%) on ectoine production were studied, and a DO control strategy was developed based on the fermentation kinetics. When the final optimized two-stage fermentation strategy was used, the ectoine titer reached 47.8 g/L, which was the highest level of ectoine produced by E. coli fermentation. The fermentation regulation strategy developed in this study might be useful for scaling up the commercial production of ectoine.
机译：在该研究中，通过从Halomonas venusta Zhiuse of Certabc操纵子构成重组胞外产生的大肠杆菌EtO1。为了进一步改善胞外生产，系统地研究了发酵过程的调节。首先，分析了初始葡萄糖浓度和葡萄糖喂养模式对胞外产生的影响。使用pH-反馈进料和葡萄糖控料的组合，胞外滴度达到25.5克/升，而是在标准分批培养上增加8.8倍。然后，研究了溶解氧（DO）水平（50,40,30或20％）对胞外产生的影响，并且基于发酵动力学开发了一种控制策略。当使用最终优化的两级发酵策略时，胞外滴度达到47.8克/升，其是大肠杆菌发酵产生的最高水平的胞外。本研究中开发的发酵调节策略可能有助于扩大卵巢的商业生产。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2021年第7期|1557-1566|共10页
  • 作者

    Dong Yingsheng; Zhang Hao; Wang XinYi; Ma JunJie; Lei Peng; Xu Hong; Li Sha;

  • 作者单位

    Nanjing Tech Univ State Key Lab Mat Oriented Chem Engn Nanjing 211816 Peoples R China|Nanjing Tech Univ Coll Food Sci & Light Ind Nanjing 211816 Peoples R China;

    Nanjing Tech Univ State Key Lab Mat Oriented Chem Engn Nanjing 211816 Peoples R China|Nanjing Tech Univ Coll Food Sci & Light Ind Nanjing 211816 Peoples R China;

    Nanjing Tech Univ Coll Food Sci & Light Ind Nanjing 211816 Peoples R China;

    Nanjing Tech Univ Coll Food Sci & Light Ind Nanjing 211816 Peoples R China;

    Nanjing Tech Univ Coll Food Sci & Light Ind Nanjing 211816 Peoples R China;

    Nanjing Tech Univ State Key Lab Mat Oriented Chem Engn Nanjing 211816 Peoples R China|Nanjing Tech Univ Coll Food Sci & Light Ind Nanjing 211816 Peoples R China|Nanjing Tech Univ Jiangsu Natl Synerget Innovat Ctr Adv Mat Nanjing Peoples R China;

    Nanjing Tech Univ State Key Lab Mat Oriented Chem Engn Nanjing 211816 Peoples R China|Nanjing Tech Univ Coll Food Sci & Light Ind Nanjing 211816 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Ectoine; Escherichia coli; Two-stage glucose feeding; Step-wise dissolved oxygen control;

    机译：Ectoine;大肠杆菌;两级葡萄糖喂养;逐步溶解氧气控制;

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