Purification of FANCD2 sub-complexes

Gang Zhi; Xiaoyong Chen; William Newcomb; Jay Brown; Oliver J. Semmes; Gary M. Kupfer

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Purification of FANCD2 sub-complexes

机译：FANCD2亚复合物的纯化

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SummaryFanconi anaemia (FA) is a recessive genetic disorder characterized by bone marrow failure, birth defects and cancer. Cells from FA patients are particularly defective in removing DNA interstrand crosslinks. We have developed a working chromatography purification scheme for FANCD2, a pivotal player in the FA DNA repair pathway, to facilitate identification of FANCD2 interacting partners. In doing so, at least three distinct FANCD2 subcomplexes were found to be present, designated as large, middle, and small complexes. The small complex is composed of tetramer of FANCD2 polypeptides, which may be the building block for other FANCD2 subcomplexes.

机译：总结范可尼贫血（FA）是一种隐性遗传性疾病，其特征是骨髓衰竭，先天缺陷和癌症。来自FA患者的细胞在去除DNA链间交联方面特别有缺陷。我们为FANCD2（FA DNA修复途径中的关键角色）开发了一种有效的色谱纯化方案，以促进FANCD2相互作用伙伴的鉴定。这样做，发现至少存在三种不同的FANCD2亚复合物，分别称为大，中和小复合物。小复合物由FANCD2多肽的四聚体组成，这可能是其他FANCD2亚复合物的基础。

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来源
《British Journal of Haematology》 |2010年第1期|p.88-92|共5页
作者
Gang Zhi; Xiaoyong Chen; William Newcomb; Jay Brown; Oliver J. Semmes; Gary M. Kupfer;
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作者单位

Department of Microbiology, University of Virginia Health System, Virginia, Charlottesville, VA;

Department of Pediatrics and Department of Pathology, Yale University Medical School New Haven, CT;

Department of Microbiology, University of Virginia Health System, Virginia, Charlottesville, VA;

Department of Microbiology, University of Virginia Health System, Virginia, Charlottesville, VA;

Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, VA, USA;

Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, VA, USA;

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Fanconi anaemia; FANCD2; chromatography purification;

机译：范可尼贫血;FANCD2;色谱纯化;

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专利

1. Expression and purification of human FANCI and FANCD2 using Escherichia coli cells [J] . Takahashi Daisuke, Sato Koichi, Shimomuki Mayo, Protein Expression and Purification . 2014,第Null期

机译：使用大肠杆菌细胞表达和纯化人FANCI和FANCD2
2. Phosphorylation of FANCD2 Inhibits the FANCD2/FANCI Complex and Suppresses the Fanconi Anemia Pathway in the Absence of DNA Damage [J] . David Lopez-Martinez, Marian Kupculak, Di Yang, Cell Reports . 2019,第10期

机译：FANCD2的磷酸化抑制了FANCD2 / FANCI复合物并抑制了DNA缺失时的范可尼贫血途径。
3. The FANCD2–FANCI complex is recruited to DNA interstrand crosslinks before monoubiquitination of FANCD2 [J] . Chih-Chao Liang, Zhuolun Li, David Lopez-Martinez, Nature Communications . 2016,第1期

机译：FANCD2–FANCI复合物在FANCD2单泛素化之前被募集到DNA链间交联
4. Protein Hydroxyl-radical Footprinting on Protein-Protein and Protein-RNA Sub-complexes of the Spliceosome [C] . Romina Hofele, Carla Schmidt, Henning Urlaub American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2011

机译：蛋白质 - 蛋白质和蛋白质-RNA亚络合物的蛋白质羟基 - 自由基剖面
5. Crystal structures of the Fanconi anemia proteins: Structure of the interstrand cross-linking repair protein Fanconi anemia protein I (FANCI); structure of the human FANCF C-terminal domain; reconstitution and crystallilzation of the sub-complexes in the Fanconi anemia core complex. [D] . Xu, Guozhou. 2009

机译：Fanconi贫血蛋白的晶体结构：链间交联修复蛋白Fanconi贫血蛋白I（FANCI）的结构；人FANCF C末端结构域的结构； Fanconi贫血核心复合物中亚复合物的重组和结晶。
6. Purification of FANCD2 Sub-complexes [O] . Gang Zhi, Xiaoyong Chen, William Newcomb, -1

机译：FANCD2亚复合物的纯化
7. FANCD2–FANCI is a clamp stabilized on DNA by monoubiquitination of FANCD2 during DNA repair [O] . Pablo Alcón, Shabih Shakeel, Zhuo A. Chen, 2020

机译：Fancd2-Fanci是在DNA修复期间由FANCD2的FANCD2稳定在DNA上的夹子

Purification of FANCD2 sub-complexes

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