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Microbial quality assessment of indoor air in a large hospital building during winter and spring seasons

机译:冬季和春季,大型医院建筑中室内空气的微生物质量评估

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Sensitivity of hospital building environment due to presence of potential sources of wide range of airborne microbes, make it a complex environment. Present study aimed to investigate seasonal (winter & spring) variation in airborne microbial levels as well as species on various locations (i.e. operation theatres (OT1 & OT2), wards (GMW & SW), out-patient department (OPD) and emergency services (ES)) of a large hospital building. Air samples were collected during peak hours, twice a week, covering one month of each season. Statistically significant variation (p 0.05) in bacterial concentrations over two seasons was found only for OPD. However, fungal concentrations significantly varied (p 0.05) over two seasons for all sites except for OT1 and OT2. Concentrations among most of sites were significantly different. Highest bacterial level was found in OPD (mean: 1649.7 CFU/m(3)) while lowest in the two OTs (mean: 221 CFU/m(3) for OT1 and 236 CFU/m(3) for OT2). Highest fungal level was found in GMW (mean: 193.4 CFU/m(3)) while lowest in the two OTs (mean: 41.1 CFU/m(3) for OT2 and 58 CFU/m(3) for OT1). Bacterial identification showed dominancy of gram positive cocci (89.8%) followed by gram positive rods (7.2%) and gram negative rods (3%). Identified bacterial strains belonged to genera staphylococcus, micrococcus, kocuria, aerococcus, kytococcus, bacillus and pseudomonas. The most abundant fungal genera included cladosporium (47%), aspergillus (17.1%), penicillium (7.1%), altemeria (6.2%), geotrichium (3.68%) and ulocladium (3.2%). Cleaning frequencies appeared to be important factor in maintaining low microbial load in air.
机译:由于存在多种空气传播微生物的潜在来源,医院建筑环境的敏感性使其成为一个复杂的环境。当前的研究旨在调查空气传播的微生物水平以及各个位置(例如手术室(OT1和OT2),病房(GMW和SW),门诊部(OPD)和急诊服务处的物种的季节性(冬季和春季)变化(ES))。每周两次,在高峰时段收集空气样本,每个季节一个月。仅在OPD中发现两个季节内细菌浓度的统计学显着变化(p> 0.05)。但是,除了OT1和OT2以外,所有地点的真菌浓度在两个季节内均存在显着变化(p <0.05)。大多数地点之间的浓度差异显着。在OPD中发现最高细菌水平(平均:1649.7 CFU / m(3)),而在两个OT中最低(在OT1中平均为221 CFU / m(3),在OT2中平均为236 CFU / m(3))。在GMW中发现最高的真菌水平(平均:193.4 CFU / m(3)),而在两个OT中发现最低的真菌水平(OT2的平均真菌含量为41.1 CFU / m(3),OT1的平均真菌含量为58 CFU / m(3))。细菌鉴定显示,革兰氏阳性球菌占优势(89.8%),其次是革兰氏阳性球菌(7.2%)和革兰氏阴性球菌(3%)。鉴定出的细菌菌株属于葡萄球菌属,微球菌属,科库里亚属,气球菌属,kycococcus属,芽孢杆菌属和假单胞菌属。最丰富的真菌属包括锁孢子菌(47%),曲霉菌(17.1%),青霉菌(7.1%),alterneria(6.2%),土t(3.68%)和(3.2%)。清洁频率似乎是保持空气中低微生物负荷的重要因素。

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