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首页> 外文期刊>World Journal of Gastroenterology >Influence of dexamethasone on inflammatory mediators and NF-κB expression in multiple organs of rats with severe acute pancreatitis
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Influence of dexamethasone on inflammatory mediators and NF-κB expression in multiple organs of rats with severe acute pancreatitis

机译：地塞米松对重症急性胰腺炎大鼠多器官炎性介质和NF-κB表达的影响

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AIM: To observe the therapeutic effects of dexamethasone on rats with severe acute pancreatitis (SAP) and investigate the influences of dexamethasone on the inflammatory mediators and NF-κB expression in multiple organs of SAP rats as well as the mechanisms involved. METHODS: Ninety Sprague-Dawley (SD) rats with SAP were randomly divided into the model group (n = 45) and dexamethasone treatment group (n = 45), and another 45 rats were selected for the sham operation group. All groups were randomly subdivided into the 3 h, 6 h and 12 h groups, each group containing 15 rats. The survival of all groups and pathological changes of multiple organs (liver, kidney and lung) were observed at different time points after the operation. The pathological score of multiple organs was carried out, followed by the determination of amylase, endotoxin and TNF-α contents in blood. The tissue microarray was used to detect the expression levels of NF-κB p65 protein in multiple organs. RESULTS: There was no marked difference between the model group and treatment group in the survival rate. The amylase content of the treatment group was significantly lower compared to the model group at 12 h (P < 0.01, 7791.00 vs 9195.00). Moreover, the endotoxin and TNF-α levels of the treatment group were significantly lower than that of the model group at 6 h and 12 h (P < 0.01, 0.040 vs 0.055, 0.042 vs 0.059 and P < 0.05, 58.30 vs 77.54, 38.70 vs 67.30, respectively). Regarding the changes in liver NF-κB expression, the model group significantly exceeded the sham operation group at 3 h (P < 0.01, 1.00 vs 0.00), and the treatment group significantly exceeded the sham operation group at 12 h (P < 0.01, 1.00 vs 0.00), whereas no marked difference was observed between the model group and treatment group at all time points. The kidney NF-κB expression level in the treatment group significantly exceeded the model group (P < 0.05, 2.00 vs 0.00) and the sham operation group (P < 0.01, 2.00 vs 0.00) at 12 h. No NF-κB expression in the lung was found in any group. CONCLUSION: Dexamethasone can lower the amylase, endotoxin and TNF-α levels as well as mortality of SAP rats. NF-κB plays an important role in multiple organ injury. Further studies should be conducted to determine whether dexamethasone can ameliorate the pathological changes of multiple organs by reducing the NF-κB expression in the liver and kidney. The advantages of tissue microarrays in pancreatitis pathological examination include time- and energy- saving, and are highly efficient and representative. The restriction of tissue microarrays on the representation of tissues to various extents due to small diameter may lead to the deviation of analysis.
机译：目的：观察地塞米松对重症急性胰腺炎（SAP）大鼠的治疗作用，探讨地塞米松对SAP大鼠多器官炎性介质和NF-κB表达的影响及其机制。方法：90只SD大鼠随机分为模型组（45只）和地塞米松治疗组（45只），另选45只假手术组。将所有组随机分为3小时，6小时和12小时组，每组包含15只大鼠。在手术后的不同时间点观察各组的存活率和多个器官（肝，肾和肺）的病理变化。进行多个器官的病理评分，然后测定血液中的淀粉酶，内毒素和TNF-α含量。组织芯片用于检测NF-κBp65蛋白在多个器官中的表达水平。结果：模型组与治疗组生存率无明显差异。与模型组相比，治疗组在12 h时的淀粉酶含量明显降低（P <0.01，7791.00对9195.00）。此外，治疗组在6 h和12 h的内毒素和TNF-α水平显着低于模型组（P <0.01，0.040 vs 0.055，0.042 vs 0.059，P <0.05，58.30 vs 77.54，38.70 vs 67.30）。关于肝脏NF-κB表达的变化，模型组在3 h时明显超过了假手术组（P <0.01，1.00 vs 0.00），治疗组在12 h时明显超过了假手术组（P <0.01， 1.00 vs 0.00），而模型组和治疗组在所有时间点均未观察到明显差异。治疗组在12 h的肾脏NF-κB表达水平显着超过模型组（P <0.05，2.00 vs 0.00）和假手术组（P <0.01，2.00 vs 0.00）。在任何一组中均未发现肺中NF-κB表达。结论：地塞米松可以降低SAP大鼠的淀粉酶，内毒素和TNF-α水平以及死亡率。 NF-κB在多器官损伤中起重要作用。应该进行进一步的研究以确定地塞米松是否可以通过降低肝脏和肾脏中的NF-κB表达来改善多器官的病理变化。组织微阵列在胰腺炎病理检查中的优势包括节省时间和能源，并且高效且具有代表性。由于小直径，组织微阵列对组织表示的限制在不同程度上可能导致分析偏差。

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