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首页> 外文期刊>World Journal of Gastroenterology >Lactobacillus plantarum inhibits epithelial barrier dysfunction and interleukin-8 secretion induced by tumor necrosis factor-α
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Lactobacillus plantarum inhibits epithelial barrier dysfunction and interleukin-8 secretion induced by tumor necrosis factor-α

机译：植物乳杆菌抑制肿瘤坏死因子-α诱导的上皮屏障功能障碍和白细胞介素8分泌

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AIM: To determine whether Lactobacillus plantarum can modify the deleterious effects of tumor necrosis factor-α (TNF-α) on intestinal epithelial cells. METHODS: Caco-2 cells were incubated with TNF-α alone or in the presence of L. plantarum. Transepithelial electrical resistance was used to measure epithelial barrier function. Interleukin 8 (IL-8) secretion by intestinal epithelial cells was measured using an ELISA. Cellular lysate proteins were immunoblotted using the anti-extracellular regulated kinase (ERK), anti-phospho-ERK and anti-IκB-α. RESULTS: A TNF-α-induced decrease in transepithelial electrical resistance was inhibited by L. plantarum. TNF-α-induced IL-8 secretion was reduced by L. plantarum. L. plantarum inhibited the activation of ERK and the degradation of IκB-α in TNF-α-treated Caco-2 cells. CONCLUSION: Induction of epithelial barrier dysfunction and IL-8 secretion by TNF-α is inhibited by L. plantarum. Probiotics may preserve epithelial barrier function and inhibit the inflammatory response by altering the signal transduction pathway.
机译：目的：确定植物乳杆菌是否可以改变肿瘤坏死因子-α（TNF-α）对肠上皮细胞的有害作用。方法：将Caco-2细胞与TNF-α单独或在植物乳杆菌存在下孵育。经上皮电阻用于测量上皮屏障功能。使用ELISA测量肠上皮细胞分泌白介素8（IL-8）。使用抗细胞外调节激酶（ERK），抗磷酸化ERK和抗IκB-α对细胞裂解液蛋白进行免疫印迹。结果：植物乳杆菌抑制了TNF-α诱导的上皮电阻的降低。植物乳杆菌减少了TNF-α诱导的IL-8分泌。植物乳杆菌抑制了TNF-α处理的Caco-2细胞中ERK的活化和IκB-α的降解。结论：植物乳杆菌可抑制TNF-α诱导的上皮屏障功能障碍和IL-8分泌。益生菌可以通过改变信号转导途径来保持上皮屏障功能并抑制炎症反应。

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