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首页> 外文期刊>World Journal of Gastroenterology >Telomerase activity in colorectal cancer, prognostic factor and implications in the microsatellite instability pathway
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Telomerase activity in colorectal cancer, prognostic factor and implications in the microsatellite instability pathway

机译：大肠癌中的端粒酶活性，预后因素及其对微卫星不稳定性途径的影响

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AIM: To determine whether the telomerase activity is related to the Microsatellite instability (MSI) genetic pathway and whether it means a difference in the survival. METHODS: The population consisted of 97 colorectal cancer patients. MSI determination was performed in accordance with the NCI criteria using PCR and Genescan. Telomerase activity was determined by the TRAP-assay, an ELISA procedure based on the amplification of telomeric repeat sequences. RESULTS: 6.2% showed high MSI (MSI-H), 10.3% showed low MSI (MSI-L) and 83.5% did not show this alteration (MSS). Positive telomerase activity was detected in 92.8% of the patients. 83.3% of MSI-H tumors showed positive telomerase against 93.8% of MSS tumors. In the overall survival analysis the absence of telomerase activity conferred a better prognosis. CONCLUSION: Previous works have shown that tumors which develop via the MSI pathway present a better prognosis. No link between telomerase activity and MSI status is observed, although sample sizes are small. Patients with telomerase negative tumors had better overall survival than patients with telomerase positive tumors.
机译：目的：确定端粒酶活性是否与微卫星不稳定性（MSI）遗传途径有关，以及它是否意味着生存差异。方法：该人群由97名结直肠癌患者组成。使用PCR和Genescan，根据NCI标准进行MSI测定。端粒酶活性通过TRAP测定法确定，TRAP测定法是基于端粒重复序列扩增的ELISA方法。结果：6.2％的患者表现出较高的MSI（MSI-H），10.3％的患者表现出较低的MSI（MSI-L），83.5％的患者未表现出这种改变（MSS）。在92.8％的患者中检测到端粒酶阳性。 83.3％的MSI-H肿瘤对93.8％的MSS肿瘤表现出端粒酶阳性。在总生存分析中，端粒酶活性的缺乏预示了更好的预后。结论：以往的研究表明，通过MSI途径发展的肿瘤预后较好。尽管样本量很小，但未观察到端粒酶活性与MSI状态之间的联系。端粒酶阴性肿瘤患者的总生存期优于端粒酶阳性肿瘤患者。

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