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首页> 外文期刊>World Journal of Gastroenterology >Role of nitric oxide in Toll-like receptor 2 and 4 mRNA expression in liver of acute hemorrhagic necrotizing pancreatitis rats.
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Role of nitric oxide in Toll-like receptor 2 and 4 mRNA expression in liver of acute hemorrhagic necrotizing pancreatitis rats.

机译：一氧化氮在急性出血性坏死性胰腺炎大鼠肝脏Toll样受体2和4 mRNA表达中的作用。

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AIM: To investigate the role of nitric oxide (NO) in Toll-like receptor 2 (TLR2)/4mRNA expression in livers of acute hemorrhagic necrotizing pancreatitis (AHNP) rats. METHODS: One hundred and ten SD male rats were randomly divided into sham-operated group (n=10), AHNP group (n=30), chloroquine (CQ)-treated group (n=30) and L-Arg-treated group (n=40). TLR2/4mRNA expression in the liver of AHNP rats was measured by RT-PCR. RESULTS: Expression of TLR2/4mRNA could be detected in the liver of AHNP rats in sham-operated group (0.155E-5+/-0.230E-6 and 0.115E-2+/-0.545E-4), but was markedly increased at 3 h in AHNP group (0.197E-2+/-0.114E-3 and 0.175+/-0.349E-2) peaking at 12 h (0.294E-2+/-0.998E-4 and 2.673+/-2.795E-2, P<0.01). Hepatic injuries were aggravated, TNF-alpha concentration in the liver was increased and NO concentration was decreased (P<0.05 or P<0.01). When TLR2/4mRNA expression was inhibited by CQ (3 h: 1.037E-4+/-3.299E-6 and 0.026+/-3.462E-3; 6 h: 1.884E-4+/-4.679E-6 and 0.108+/-6.115E-3; 12 h: 2.443E-4+/-7.714E-6 and 0.348+/-6.807E-3; P<0.01), hepatic injuries were relieved, NO concentration in the liver was increased and TNF-alpha concentration was decreased (P<0.05 or P<0.01). When rats with AHNP were treated with L-Arg, TLR2/4mRNA expression in the liver could be effectively inhibited (50 mg-T: 0.232E-2+/-0.532E-4 and 0.230+/-6.883E-3; 100 mg-T: 0.210E-2+/-1.691E-4 and 0.187+/-0.849E-2; 200 mg-T: 0.163E-2+/-0.404E-4 and 0.107+/-0.195E-2; 400 mg-T: 0.100E-2+/-0.317E-4 and 0.084+/-0.552E-2; P<0.01) and hepatic injuries were relieved. At the same time, NO concentration in the liver was markedly increased and TNF-alpha concentration was decreased (P<0.05 or P<0.01). CONCLUSION: The expression of TLR2/4mRNA is increased and hepatic injuries are aggravated in the liver of AHNP rats. TLR2/4mRNA gene expression in the liver of AHNP rats can be markedly inhibited by NO, leading to the relief of hepatic injuries.
机译：目的：探讨一氧化氮（NO）在急性出血性坏死性胰腺炎（AHNP）大鼠肝脏中Toll样受体2（TLR2）/ 4mRNA表达中的作用。方法：将110只SD雄性大鼠随机分为假手术组（n = 10），AHNP组（n = 30），氯喹（CQ）治疗组（n = 30）和L-Arg治疗组（n = 40）。 RT-PCR检测AHNP大鼠肝脏中TLR2 / 4mRNA的表达。结果：假手术组（0.155E-5 +/- 0.230E-6和0.115E-2 +/- 0.545E-4）在AHNP大鼠肝脏中可检测到TLR2 / 4mRNA表达，但其表达明显AHNP组（0.197E-2 +/- 0.114E-3和0.175 +/- 0.349E-2）在3小时时增加，在12小时（0.294E-2 +/- 0.998E-4和2.673 +/-）达到峰值2.795E-2，P ＜0.01）。肝损伤加重，肝脏中TNF-α浓度升高，NO浓度降低（P <0.05或P <0.01）。当CLR抑制TLR2 / 4mRNA表达时（3小时：1.037E-4 +/- 3.299E-6和0.026 +/- 3.462E-3; 6小时：1.884E-4 +/- 4.679E-6和0.108 +/- 6.115E-3; 12 h：2.443E-4 +/- 7.714E-6和0.348 +/- 6.807E-3; P <0.01），减轻了肝损伤，增加了肝脏中的NO浓度， TNF-α浓度降低（P <0.05或P <0.01）。用L-Arg治疗AHNP大鼠时，肝脏中TLR2 / 4mRNA的表达可被有效抑制（50 mg-T：0.232E-2 +/- 0.532E-4和0.230 +/- 6.883E-3； 100 mg-T：0.210E-2 +/- 1.691E-4和0.187 +/- 0.849E-2； 200 mg-T：0.163E-2 +/- 0.404E-4和0.107 +/- 0.195E-2 ; 400 mg-T：0.100E-2 +/- 0.317E-4和0.084 +/- 0.552E-2; P <0.01）和肝损伤得到缓解。同时，肝脏中的NO浓度显着升高，TNF-α浓度降低（P <0.05或P <0.01）。结论：AHNP大鼠肝脏TLR2 / 4mRNA表达升高，肝损伤加重。 NO可以明显抑制AHNP大鼠肝脏中的TLR2 / 4mRNA基因表达，从而减轻肝损伤。

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