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首页> 外文期刊>World Journal of Gastroenterology >Role of transforming growth factor-beta1-smad signal transduction pathway in patients with hepatocellular carcinoma.
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Role of transforming growth factor-beta1-smad signal transduction pathway in patients with hepatocellular carcinoma.

机译：转化生长因子-β1-smad信号转导通路在肝细胞癌患者中的作用。

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AIM: To explore the role of transforming growth factor-beta1 (TGF-beta1)-smad signal transduction pathway in patients with hepatocellular carcinoma. METHODS: Thirty-six hepatocellular carcinoma specimens were obtained from Qidong Liver Cancer Institute and Department of Pathology of the Second Affiliated Hospital of Nanjing Medical University. All primary antibodies (polyclonal antibodies) to TGF-beta1, type II Transforming growth factor-beta receptor (TbetaR-II), nuclear factor-kappaB (NF-kappaB), CD34, smad4 and smad7,secondary antibodies and immunohistochemical kit were purchased from Zhongshan Biotechnology Limited Company (Beijing, China). The expressions of TGF-beta1, TbetaR-II, NF-kappaB, smad4 and smad7 proteins in 36 specimens of hepatocellular carcinoma (HCC) and its adjacent tissue were separately detected by immunohistochemistry to observe the relationship between TGF-beta1 and TbetaR-II, between NF-kappaB and TGF-beta1, between smad4 and smad7 and between TGF-beta1 or TbetaR-IIand microvessel density (MVD). MVD was determined by labelling the vessel endothelial cells with CD34. RESULTS: The expression of TGF-beta1, smad7 and MVD was higher in HCC tissue than in adjacent HCC tissue (P<0.01, P<0.05, P<0.01 respectively). The expression of TbetaR-IIand smad4 was lower in HCC tissue than in its adjacent tissue (P<0.01, P<0.05 respectively). The expression of TGF-beta1 protein and NF-kappaB protein was consistent in HCC tissue. The expression of TGF-beta1 and MVD was also consistent in HCC tissue. The expression of TbetaR-IIwas negatively correlated with that of MVD in HCC tissue. CONCLUSION: The expressions of TGF-beta1, TbetaR-II, NF-kappaB, smad4 and smad7 in HCC tissue, which are major up and down stream factors of TGF-beta1-smad signal transduction pathway , are abnormal. These factors are closely related with MVD and may play an important role in HCC angiogenesis. The inhibitory action of TGF-beta1 is weakened in hepatic carcinoma cells because of abnormality of TGF-beta1 receptors (such as TbetaR-II) and postreceptors (such as smad4 and smad7). NF-kappaB may cause activation and production of TGF-beta1.
机译：目的：探讨转化生长因子-β1（TGF-β1）-smad信号转导通路在肝细胞癌患者中的作用。方法：36例肝细胞癌标本取自启东市肝癌研究所和南京医科大学附属第二医院病理科。所有的TGF-beta1，II型转化生长因子-beta受体（TbetaR-II），核因子-κB（NF-kappaB），CD34，smad4和smad7的一抗（多克隆抗体），二抗和免疫组化试剂盒购自中山生物技术有限公司（中国北京）。免疫组织化学分别检测36例肝细胞癌（HCC）及其附近组织中TGF-beta1，TbetaR-II，NF-κB，smad4和smad7的表达，观察TGF-beta1与TbetaR-II的关系， NF-κB和TGF-beta1之间，smad4和smad7之间以及TGF-beta1或TbetaR-II之间和微血管密度（MVD）之间。通过用CD34标记血管内皮细胞来确定MVD。结果：肝癌组织中TGF-β1，smad7和MVD的表达高于癌旁组织（P <0.01，P <0.05，P <0.01）。肝癌组织中TbetaR-II和smad4的表达低于癌旁组织（P <0.01，P <0.05）。在肝癌组织中TGF-beta1蛋白和NF-κB蛋白的表达是一致的。在肝癌组织中，TGF-beta1和MVD的表达也一致。在肝癌组织中，TbetaR-II的表达与MVD呈负相关。结论：TGF-β1，TbetaR-II，NF-κB，smad4和smad7在肝癌组织中的表达异常，这是TGF-β1-smad信号转导途径的主要上游和下游因素。这些因素与MVD密切相关，并且可能在HCC血管生成中起重要作用。由于TGF-β1受体（例如TbetaR-II）和后受体（例如smad4和smad7）的异常，TGF-β1在肝癌细胞中的抑制作用减弱。 NF-κB可能引起TGF-beta1的激活和产生。

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