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首页> 外文期刊>World Journal of Gastroenterology >Involvement of mitochondrial Na+-Ca2+ exchange in intestinal pacemaking activity.
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Involvement of mitochondrial Na+-Ca2+ exchange in intestinal pacemaking activity.

机译：线粒体Na + -Ca2 +交换参与肠道起搏活动。

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摘要

AIM: Interstitial cells of Cajal (ICCs) are the pacemaker cells that generate slow waves in the gastrointestinal (GI) tract. We have aimed to investigate the involvement of mitochondrial Na+-Ca2+ exchange in intestinal pacemaking activity in cultured interstitial cells of Cajal. METHODS: Enzymatic digestions were used to dissociate ICCs from the small intestine of a mouse. The whole-cell patch-clamp configuration was used to record membrane currents (voltage clamp) and potentials (current clamp) from cultured ICCs. RESULTS: Clonazepam and CGP37157 inhibited the pacemaking activity of ICCs in a dose-dependent manner. Clonazepam from 20 to 60 micromol/L and CGP37157 from 10 to 30 micromol/L effectively inhibited Ca2+ efflux from mitochondria in pacemaking activity of ICCs. The IC50s of clonazepam and CGP37157 were 37.1 and 18.2 micromol/L, respectively. The addition of 20 micromol/L NiCl2 to the internal solution caused a "wax and wane" phenomenon of pacemaking activity of ICCs. CONCLUSION: These resultssuggest that mitochondrial Na+-Ca2+ exchange has an important role in intestinal pacemaking activity.
机译：目的：Cajal间质细胞（ICC）是起搏器细胞，可在胃肠道（GI）中产生慢波。我们的目的是调查培养的Cajal间质细胞中线粒体Na + -Ca2 +交换参与肠道起搏活动。方法：使用酶消化法从小鼠小肠中分离出ICC。全细胞膜片钳配置用于记录培养的ICC的膜电流（电压钳）和电势（电流钳）。结果：氯硝西am和CGP37157以剂量依赖性方式抑制ICC的起搏活性。 20至60微摩尔/升的氯硝西and和10至30微摩尔/升的CGP37157有效抑制ICCs的起搏活动中线粒体的Ca2 +外流。氯硝西am和CGP37157的IC50分别为37.1和18.2 micromol / L。向内部溶液中添加20 micromol / L NiCl2会导致ICC的起搏活动出现“蜡和蜡”现象。结论：这些结果表明线粒体Na + -Ca 2+交换在肠道起搏活动中具有重要作用。

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