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首页> 外文期刊>World Journal of Gastroenterology >Role of CD80 in stimulating T lymphocyte activation.
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Role of CD80 in stimulating T lymphocyte activation.

机译：CD80在刺激T淋巴细胞活化中的作用。

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AIM:To observe biological characteristics of hepatocarcinoma cells before and after CD80 transfection and to compare the effect of CD80-transfected hepatocarcinoma cells on T lymphocyte activation.METHODS:Retro virus vector carrying CD80 gene was transfected into HepG2 cells to establish CD80-transfected hepatocarcinoma cells (HepG2/hCD80). Flow cytometry (FCM) was performed to detect CD80 expression in the transfected cells. RT-PCR was used to evaluate CD80 expression at mRNA level. In the presence of anti-CD3 mAb, the proliferation of T lymphocyte was observed by MTT. Meanwhile, the expression of activated molecule marker CD25 was analyzed through FCM.RESULTS:A stable cell line HepG2/hCD80 expressing the human CD80 was established. Growth curve showed that the molecule CD80 could obviously decrease the growth of tumor cells. HepG2/hCD80 was evidenced to have a potency to enhance T cell proliferation and upregulate CD25 expression.CONCLUSION:CD80 transfection can lower malignant phenotype of hepatocarcinoma cells. CD80 transfection has a down-regulatory effect to activated T cells in vitro.
机译：目的：观察CD80转染前后肝癌细胞的生物学特性，比较CD80转染的肝癌细胞对T淋巴细胞活化的影响。方法：将携带CD80基因的逆转录病毒载体转染HepG2细胞，建立CD80转染的肝癌细胞（HepG2 / hCD80）。进行流式细胞术（FCM）以检测转染细胞中的CD80表达。 RT-PCR用于评估mRNA水平的CD80表达。在抗CD3mAb的存在下，MTT观察到T淋巴细胞的增殖。同时，通过流式细胞仪分析了活化分子标志物CD25的表达。结果：建立了表达人CD80的稳定细胞系HepG2 / hCD80。生长曲线表明，分子CD80可以明显抑制肿瘤细胞的生长。证明HepG2 / hCD80具有增强T细胞增殖和上调CD25表达的能力。结论：CD80转染可降低肝癌细胞的恶性表型。 CD80转染对体外活化的T细胞有下调作用。

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