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首页> 外文期刊>World Journal of Gastroenterology >Eubacterium limosum ameliorates experimental colitis and metabolite of microbe attenuates colonic inflammatory action with increase of mucosal integrity.
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Eubacterium limosum ameliorates experimental colitis and metabolite of microbe attenuates colonic inflammatory action with increase of mucosal integrity.

机译：利莫杆菌可改善实验性结肠炎，微生物的代谢产物可通过增加粘膜完整性来减弱结肠的炎症作用。

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AIM: To examine the effect of Eubacterium limosum (E. limosum) on colonic epithelial cell line in vitro, and to evaluate the effect of E. limosum on experimental colitis. METHODS: E. limosum was inoculated anaerobically and its metabolites were obtained. The growth stimulatory effect of the E. limosum metabolites on T84 cells was evaluated by SUDH activity, and the anti-inflammatory effect by IL-6 production. The change in mRNA of toll like receptor 4 (TLR4) was evaluated by real time PCR. Colitis was induced by feeding BALB/C mice with 2.0% dextran sodium sulfate. These mice received either 5% lyophilized E. limosum (n = 7) or control diet (n = 7). Seven days after colitis induction, clinical and histological scores, colon length, and cecal organic acid levels were determined. RESULTS: The E. limosum produced butyrate, acetate, propionate, and lactate at 0.25, 1.0, 0.025 and 0.07 mmol/L, respectively in medium. At this concentration, each acid had no growth stimulating activity on T84 cells; however, when these acids were mixed together at the above levels, it showed significantly high activity than control. Except for lactate, these acids significantly attenuated IL-6 production at just 0.1 mmol/L. In addition, under TNF-alpha stimulation, butyrate attenuated the production of TLR4 mRNA. The treatment with E. limosum significantly attenuated clinical and histological scores of colitis with an increase of cecal butyrate levels, compared with the control group. CONCLUSION: E. limosum can ameliorate experimental colonic inflammation. In part, the metabolite of E. limosum, butyrate, increases mucosal integrity and shows anti-inflammatory action modulation of mucosal defense system via TLR4.
机译：目的：探讨金黄色葡萄球菌（E.bacterium limosum）对结肠上皮细胞系的体外作用，并评估金黄色葡萄球菌对实验性结肠炎的作用。方法：厌氧接种大肠埃希菌并获得其代谢产物。通过SUDH活性评估了E. limosum代谢产物对T84细胞的生长刺激作用，并通过产生IL-6评估了其抗炎作用。通过实时PCR评估收费样受体4（TLR4）的mRNA的变化。通过给BALB / C小鼠饲喂2.0％的葡聚糖硫酸钠来诱发结肠炎。这些小鼠接受5％冻干的大肠杆菌（n = 7）或对照饮食（n = 7）。诱导结肠炎后7天，测定临床和组织学评分，结肠长度和盲肠有机酸水平。结果：大肠埃希菌在培养基中产生的丁酸酯，乙酸酯，丙酸酯和乳酸分别为0.25、1.0、0.025和0.07 mmol / L。在此浓度下，每种酸对T84细胞均无刺激生长的活性。然而，当这些酸以上述水平混合在一起时，它显示出比对照明显更高的活性。除乳酸外，这些酸仅以0.1 mmol / L的浓度即可显着降低IL-6的产生。另外，在TNF-α刺激下，丁酸酯减弱了TLR4 mRNA的产生。与对照组相比，用大肠埃希菌治疗可显着减轻结肠炎的临床和组织学评分，同时增加盲肠丁酸水平。结论：百合可改善实验性结肠炎。部分地，丁香大肠杆菌的代谢产物丁酸增加了粘膜的完整性，并通过TLR4调节了粘膜防御系统的抗炎作用。

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