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首页> 外文期刊>World Journal of Gastroenterology >Identification of a new target region on the long arm of chromosome 7 in gastric carcinoma by loss of heterozygosity.
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Identification of a new target region on the long arm of chromosome 7 in gastric carcinoma by loss of heterozygosity.

机译：通过杂合性丧失鉴定胃癌7号染色体长臂上的新靶标区域。

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摘要

AIM: To define the common deleted region on the long arm of haman chromosome 7q linked to primary gastric carcinomas in Chinese by loss of heterozygosity (LOH) and its clinical significance.METHODS: Nine microsatellite markers distributed over chromosome 7q with an average marker density of 10cM were used to examine 70 primary gastric carcinomas for LOH by PCR amplification. The PCR products were separated by electrophoresis on polyacrylamide gel. Genescan and Genotyper softwares were used to analyze LOH.RESULTS: LOH with at least one marker on 7q occurred in 34.3% (12/50) of the tumors. Among them, LOH at D7S486 and D7S798 was higher in 24.0% (24/70) and 19.2% (5/26), respectively. By statistical analysis we also observed an obvious genotype-phenotype correlation on 7q (P<0.05). The frequency of LOH at D7S486 in patients with lymph node metastasis was significantly higher than that in those without lymph node metastasis (P=0.015).CONCLUSION: The high incidence of LOH at D7S486 and its correlation with poorer prognosis suggest that there might be putative tumor suppressor genes in this region involved in the tumorigenesis and progression of gastric carcinoma.
机译：目的：通过杂合性缺失（LOH）确定与中国原发性胃癌相关的哈曼染色体7q长臂上常见的缺失区及其临床意义。方法：在染色体7q上分布9个微卫星标记，平均标记密度为10cM用于通过PCR扩增检查70例原发性胃癌的LOH。通过在聚丙烯酰胺凝胶上的电泳分离PCR产物。结果：在3q％（12/50）的肿瘤中出现了至少在7q带有一种标记的LOH。其中，D7S486和D7S798的LOH分别较高，分别为24.0％（24/70）和19.2％（5/26）。通过统计分析，我们还在7q上观察到了明显的基因型-表型相关性（P <0.05）。有淋巴结转移的患者D7S486的LOH发生频率明显高于无淋巴结转移的患者（P = 0.015）。结论：D7S486的LOH发生率高且与预后较差相关，提示可能该区域中的肿瘤抑制基因参与胃癌的发生和发展。

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