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首页> 外文期刊>World Journal of Gastroenterology >Modulatory effects of Azadirachta indica on benzo(a)pyrene-induced forestomach tumorigenesis in mice.
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Modulatory effects of Azadirachta indica on benzo(a)pyrene-induced forestomach tumorigenesis in mice.

机译：印za对小鼠苯并（a）py诱导的前胃肿瘤的调节作用。

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AIM: To evaluate the chemopreventive effects of aqueous Azadirachta indica (A indica) leaf extract (AAILE) against benzo(a)pyrene [B(a)P]-induced forestomach tumorigenesis in Balb/c mice. METHODS: Female Balb/c mice were divided into four groups of 10-12 animals each. For induction of forestomach tumors, starting from d 14 of the experi-ment, mice of B(a)P and B(a)P+A indica groups were given intra-gastric instillations of B(a)P (40 mg/kg), twice a week for four weeks. Mice of A indica and B(a)P+A indica groups were orally administered with AAILE (100 mg/kg), two weeks prior to B(a)P instillations till the end of the experiment. After 22 wk of the first B(a)P instillation, mice were sacrificed and the forestomachs were analyzed for development of tumors, scanning electron microscopy (SEM) and histopathology. RESULTS: Tumor incidence was observed to be 100% in mice that received only B(a)P. However, treatment with AAILE reduced the tumor incidence by 58.4% as observed in mice of B(a)P+A indica group when compared to that of B(a)P group. Similarly, the tumor burden and multiplicity were seen to decrease by 87.3% and 69.6% respectively in mice of B(a)P+A indica group when compared to those of B(a)P group. Scanning electron microscopy analysis showed that AAILE treatment itself did not cause any abnormalities on the surface architecture of forestomach epithelium. In tumorous forestomach, surface disruption was observed. Over the forestomach tumors of B(a)P group of mice certain rounded structures were seen in addition to closely placed tongue-shaped squamous cells. Interestingly, these rounded structures were not observed in B(a)P + A indica group of mice. Histopathalogically, the tumors were identical and diagnosed to be papillomas. Mice from control and A indica groups of mice did not develop any forestomach tumors and showed normal histo-architecture. CONCLUSION: The present data suggest that A indica exerts chemopreventive effects against B(a)P-induced forestomach tumors in murine model. Because of lack of toxicity and ubiquitous bioavailability, A indica may play a promising role in future drug discovery and development as far as chemoprevention of cancer is concerned.
机译：目的：评估印A叶提取物（AAILE）对Balb / c小鼠中苯并[a] py [B（a）P]诱导的前胃癌发生的化学预防作用。方法：将雌性Balb / c小鼠分为四组，每组10-12只动物。为了诱导前胃癌肿瘤，从实验的第14天开始，对B（a）P和B（a）P + A d型组的小鼠进行胃内滴注B（a）P（40 mg / kg ），每周两次，共四个星期。在将B（a）P滴注至实验结束前两周，对A d和B（a）P + A d组的小鼠口服AAILE（100 mg / kg）。第一次B（a）P滴注22周后，处死小鼠，并分析前胃管的肿瘤发展，扫描电子显微镜（SEM）和组织病理学。结果：仅接受B（a）P的小鼠的肿瘤发生率为100％。但是，与B（a）P组相比，用AAILE治疗可以使B（a）P + A indica组小鼠的肿瘤发生率降低58.4％。同样，与B（a）P组相比，B（a）P + A group型组小鼠的肿瘤负担和多重性分别降低了87.3％和69.6％。扫描电子显微镜分析表明，AAILE治疗本身并未在前胃上皮的表面结构上引起任何异常。在肿瘤前胃中，观察到表面破坏。在B（a）P组小鼠的前胃肿瘤上，除了紧密放置的舌状鳞状细胞外，还观察到某些圆形结构。有趣的是，在B（a）P + A indica组的小鼠中未观察到这些圆形结构。在组织病理学上，肿瘤是相同的，并被诊断为乳头状瘤。来自对照组和A d小鼠组的小鼠没有发展成任何前胃瘤肿瘤，并且显示出正常的组织结构。结论：目前的数据表明，印度in对小鼠模型中B（a）P诱导的前胃肿瘤具有化学预防作用。由于缺乏毒性和普遍存在的生物利用度，就化学预防癌症而言，in可能在未来的药物研发中发挥有前途的作用。

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