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首页> 外文期刊>World Journal of Gastroenterology >Dynamic functional and ultrastructural changes of gastric parietal cells induced by water immersion-restraint stress in rats.
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Dynamic functional and ultrastructural changes of gastric parietal cells induced by water immersion-restraint stress in rats.

机译：浸水抑制应激诱导的大鼠胃壁细胞动态功能和超微结构变化。

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摘要

AIM: To investigate the dynamic functional and ultrastructural changes of gastric parietal cells induced by water immersion-restraint stress (WRS) in rats. METHODS: WRS model of Sprague-Dawley (SD) rats was established. Fifty-six male SD rats were randomly divided into control group, stress group and post-stress group. The stress group was divided into 1, 2 and 4 h stress subgroups. The post-stress group was divided into 24, 48 and 72 h subgroups. The pH value of gastric juice, ulcer index (UI) of gastric mucosa and H(+), K(+)-ATPase activity of gastric parietal cells were measured. Ultrastructural change of parietal cells was observed under transmission electron microscope (TEM). RESULTS: The pH value of gastric juice decreased time-dependently in stress group and increased in post-stress group. The H(+), K(+)-ATPase activity of gastric parietal cells and the UI of gastric mucosa increased time-dependently in stress group and decreased in post-stress group. Compared to control group, the pH value decreased remarkably (P = 0.0001), the UI and H(+), K(+)-ATPase activity increased significantly (P = 0.0001, P = 0.0174) in 4 h stress subgroup. UI was positively related with stress time (r = 0.9876, P < 0.01) but negatively with pH value (r = -0.8724, P < 0.05). The parietal cells became active in stress group, especially in 4 h stress subgroup, in which plenty of intracellular canalicular and mitochondria were observed under TEM. In post-stress group, the parietal cells recovered to resting state. CONCOUSION: The acid secretion of parietal cells is consistent with their ultrastructural changes during the development and healing of stress ulcer induced by WRS and the degree of gastric mucosal lesions, suggesting gastric acid play an important role in the development of stress ulcer and is closely related with the recovery of gastric mucosal lesions induced by WRS.
机译：目的：探讨水浸约束应激（WRS）诱导大鼠胃壁细胞动态功能和超微结构的变化。方法：建立SD大鼠的WRS模型。 56只SD雄性大鼠随机分为对照组，应激组和应激后组。应激组分为1、2和4 h应激亚组。压力后组分为24、48和72小时亚组。测量胃液pH值，胃黏膜溃疡指数（UI）和胃壁细胞H（+），K（+）-ATPase活性。在透射电镜下观察壁细胞的超微结构变化。结果：应激组胃液pH值随时间呈下降趋势，应激后组胃酸pH升高。应激组胃壁细胞的H（+），K（+）-ATPase活性和胃粘膜UI呈时间依赖性增加，应激后组呈下降趋势。与对照组相比，在4 h应激亚组中，pH值显着降低（P = 0.0001），UI和H（+），K（+）-ATPase活性显着提高（P = 0.0001，P = 0.0174）。 UI与应激时间呈正相关（r = 0.9876，P <0.01），而与pH值呈负相关（r = -0.8724，P <0.05）。在应激组，特别是在4 h应激亚组中壁细胞变得活跃，其中在TEM下观察到大量细胞内小管和线粒体。应激后组壁细胞恢复到静止状态。结论：在WRS引起的应激性溃疡的发生和愈合过程中，壁细胞的酸分泌与其超微结构的变化以及胃黏膜病变的程度有关，提示胃酸在应激性溃疡的发生中起着重要的作用，并与之密切相关。与WRS引起的胃粘膜病变的恢复有关。

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